Real-time PCR System

Used to assess gene expression and perform genotyping, copy number determination, microRNA analyses, and pathogen detection. The system is equipped with interchangeable blocks that are controlled with a Dell Desktop computer containing QuantStudio 6 and 7 Software as well as Primer Express Software. The 384-well format increases throughput and reduces reagent usage; the 96-well Fast block reduces real-time PCR runs from 2 hours to 40 minutes, which will allow 3 times as many experiments to be run each day; and blocks have three temperature zones. Both SYBR Green and TaqMan reactions can be run, with 5 coupled excitation and emission filter channels.