BCH Cores Specialized Equipment Listing

The PromethION 24 by Oxford Nanopore Technologies offers scalable and versatile long-read sequencing that captures native DNA and RNA fragments ranging from 20 to four million bases. The system supports up to 24 simultaneous experiments rapid workflows and real-time data analysis, ideal for whole genome sequencing, methylation analysis, or direct RNA sequencing. The PromethION 24 delivers high-yield, biologically-dense genomes that satisfy a wide range of sequencing interests.

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Machine learning analysis of bottom-up imaging of rodent behavior.

For fully automated nucleic acid extraction with QIAGEN spin-column kits. Automates over 80 QIAGEN kits with over 3000 protocols. Allows automated DNA, RNA and protein sample processing. Eliminates manual processing steps. Generates standardized results.

Large scale magnetic cell selection

Cell culture bioprocess analyzer (glucose, lactate etc)

Molecular monitoring of patients on gene therapy trials

Cell sorting for therapy applications

Multiplexing analytes

The Symphony is a 6-laser flow cytometry with the flexibility to sort up to 6 ways or into plates or slides. This cytometer helps to isolate subpopulations and can measure 20 or more colors.

3D/4D visualization and analysis for cell based segmentation, automated tracking, detection, interactive lineage trees and statistical tests (for customization in Matlab, Java or Python)

CO2 and Temp control for live cell monitoring on Keyence epifluorescent scope

Generates individually barcoded sequencing libraries for hundreds of thousands of nanoliter volume oil droplets using up to 1.7 million different barcodes

Microscope Camera

Provide incubation to DiSPIM microscope

Mammalian cell/tissue culture

Mammalian cell/tissue culture

The Singulator 100 system automates the isolation and processing of single cells from tissue samples via microfluidic technology. Maintaining cell viability and integrity, it offers gentle extraction of cells from heterogeneous samples enabling downstream analysis including single cell sequencing and transcriptomics.

The Covaris R230 Focused-ultrasonicator is designed for precise and controlled shearing of biological samples. It delivers highly reproductible and efficient sample processing to achieve targeted fragment sizes across a wide range of sample types. This system is ideal for several applications including DNA and chromatin shearing, next-generation sequencing library preparation, and chromatin immunoprecipitation (ChIP).

The 10x Genomics Chromium X Series enables single-cell and spatial genomics research via microfluidic technology and parallel sequencing capabilities. For low and high throughput, the Chromium iX handles all single-cell assays and analyzes hundreds of thousands of cells per run.

The SeqStudio Flex Series is a 24 capillary electrophoresis system capable of Sanger Sequencing analysis (for NGS confirmation, indel detection, SNP detection, genome editing verification, microbial identification, etc…) and fragment analysis (ie microsatellite analysis).

Ultimate 3000 HPLC with UV/Vis, florescent and electrochemical detectors and Chromeleon analysis software. This is a versatile HPLC system with multiple detectors to quantify a variety of analytes of interest.

In vivo electroporation of mouse embryos

This item was funded in ECRAC cycle 17 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 17 and has not yet been purchased. Please contact the core for more information.

A spinning disk confocal microscope is used for high-throughput, high-content assays, phenotypic screening, assays using complex disease models, such as live cells, primary cells, and microtissues, and fast-response assays, such as Ca2+ flux. It is equipped with a liquid handling environmental chamber, 5 lasers and 4 cameras, a full set of objectives, 5x air, 10x air, 20x air, 20x water, 40x water, and 63x water objectives.

This item was funded in ECRAC cycle 16 and has not yet been purchased. Please contact the core for more information.

Scalable electroporator primarily used for gene editing (clinical)

This item was funded in ECRAC cycle 15 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 15 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 14 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 14 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 14 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.

Measure Fluorescence Spectra

A laser scanning confocal microscope enables full 3D imaging of both live and fixed samples. The airyscan detector provides faster image acquisition and higher resolution than typical confocal imaging.

This item was funded in ECRAC cycle 12 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 12 and has not yet been purchased. Please contact the core for more information.

The LUNA-FL Cell Counter fluorescence optics and image analysis for precise cell counting and viability analysis. Using two fluorescence channels and automated analysis, it simultaneously detects multiple stains for live and dead cells.

Hardware for cloud computing

This piece of equipment was funded by ECRAC in cycle 1. Please contact the core for more information.

This piece of equipment was funded by ECRAC in cycle 1. Please contact the core for more information.

This piece of equipment was funded in ECRAC cycle 3. Please contact the core for more information.

Works with 50 mL conical tubes and 1.5 ml microcentrifuge tubes.

This piece of equipment was funded in ECRAC cycle 6 and has not yet been purchased. Please contact the core for more information.

The Countess™ II FL Automated Cell Counter is a fully automated, 3-channel cell counter and assay platform that uses EVOS™ light cube technology, state-of-the-art optics, and image analysis algorithms to analyze fluorescently labeled cells or trypan blue stained samples in suspension.

This electrophysiology rig with light microscope is capable of single-cell (current-clamp, patch-clamp) and extracellular (local field potential) recordings. Additionally, this rig has two headstages, enabling dual channel recordings, and a temperature control system, allowing for physiological temperatures to be maintained during experiments. The Olympus light microscope with differential interference contrast (DIC) allows optimal visualization of specimens to confirm electrode placement. Lastly, the Lumenera camera allows for images and continuous video (30 fps at full resolution of 1280 x 1024) for documentation during recordings.

The Thermo Scientific Savant SpeedVac uses vacuum technology to expedite drying processes and efficiently concentrate and evaporate samples.

This piece of equipment was funded in ECRAC cycle 9 and has not yet been purchased. Please contact the core for more information.

This piece of equipment was funded in ECRAC cycle 9 and has not yet been purchased. Please contact the core for more information.

Deconvolution images

Dual-energy CT

The Zetaview small particle analyzer employs Nanoparticle Tracking Analysis (NTA) which relates the rate of Brownian motion of the particles in liquid suspension to particle size.

Used to assess gene expression and perform genotyping, copy number determination, microRNA analyses, and pathogen detection. The system is equipped with interchangeable blocks that are controlled with a Dell Desktop computer containing QuantStudio 6 and 7 Software as well as Primer Express Software. The 384-well format increases throughput and reduces reagent usage; the 96-well Fast block reduces real-time PCR runs from 2 hours to 40 minutes, which will allow 3 times as many experiments to be run each day; and blocks have three temperature zones. Both SYBR Green and TaqMan reactions can be run, with 5 coupled excitation and emission filter channels.

Partitioning device used to isolate single cells from a cell suspension in “Gel Bead-in-Emulsion” droplets, where nucleic acid of interest is extracted and tagged with a cell-specific barcode for downstream Next-Gen Sequencing library prep and sequencing.

Microvolume UV-Vis and Fluorescence spectrophotometer used for nucleic acid and protein quantification and quality assessment. Additionally capable of Microarray fluorescent labeling QC analysis, as well as Pierce 660, Bradford, BCA, Lowry assays. 8000 model has 8 channels improving speed.

Works with 50 mL conical tubes and 1.5 ml microcentrifuge tubes

Analyzer measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. These rates are key indicators of mitochondrial respiration and glycolysis. They provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.

Analysis of cell surface or intracellular proteins or other “markers,” intracellular cytokine production and receptor-mediated endocytosis, sorting, and recycling.

High content live cell imager capable of electrical or optical stimulation and high-speed acquisition with an analysis package designed for quantifying parameters from individual cells within a well

Epifluorescent, brightfield, and phase imaging, image stitching for capturing images larger than one field, automated multiwell plate image sample, structured illumination, live cell imaging with video rate capture and environmental controls and built-in image analysis functions.

Upright microscope with a Pixelink PL-D682CU color CMOS camera for brightfield and epifluorescence imaging.

Laser Scanning Confocal microscope with 6 laser lines (405/458/488/514/561/633nm), Fast Airyscan for near super-resolution confocal imaging, LED epifluorescence and halogen brightfield light sources, temperature controlled enclosure, motorized XY stage, piezo-controlled Z-stage, enabled for confocal, brightfield, and DIC, fixed and live cell imaging.

Confocal imaging of fixed or live samples

High-throughout high-content confocal fluorescence imaging.

functional MRI data collection

BL2 Cell Sorter

Flow Cytometry Analyzer with Plate Reader

Light Sheet Microscope, primary niche is rapid low light dose imaging of 3D objects.

Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence.

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The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.

The Saphyr System by Bionano Genomics is a whole genome imaging tool for high-speed, high-throughput structural variant detection and analysis with exceptional sensitivity and specificity. Wide range of applications includes: undiagnosed genetic disorders, hematologic malignancies, gene discovery and therapy, cell line stability, solid tumor research, genetic engineering studies, evolutionary biology, and reference genome assembly.  Resolve large-scale structural variations missed by next-generation sequencing systems. Detect structural variants with high specificity and sensitivity.

Lecia/Zeiss

Microdialysis

Microendoscope fitted with fiber-coupled laser and LED light sources that is used in conjunction with implantable gradient-index (GRIN) lenses

Conscious, unrestrained ECG

Seizure susceptibility, epileptiform abnormalities, circadian disturbances, actigraphy, auditory evoked response potentials; continuous monitoring available for up to 4 months

Attention/working memory/flexibility

Cognitive flexibility.

Short term working memory

Communications

Response to Stress

Emotional response to stimulus/condition

Anxiety/Social Recognition

Anxiety/Cognition

Motor Strength

Forced Activity

Sensorimotor Gating

Visual acuity (brain stem reflex)

Voluntary Activity

This Fortessa is an air-cooled four-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 12 colors + 2 Scatters. Hydrodynamically focused sample is interrogated within a quartz cuvette flow cell. Sample Aspiration rates at 12ul, 35ul, 60ul per minute.

This LSRFortessa is an air-cooled five-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 15 colors + 2 Scatters. They are compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates.

The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.

The FACSAria cell sorter provides customized speed sorting. This instrument is bl2 certified and equipped with an Aerosol Management option for operator protection.  Well designed for your research needs, the FACSAria allows simultaneous sorting 4 populations.  Collection can be done in tubes or Plate.  Index sorting now provides the option of match single cell profile with Fluorescence Intensity. 

CGH & CGH+SNP Microarray Scanner. The Agilent SureScan Scanner provides a precise and efficient microarray scanner system for many research applications. Designed for research using microarrays, the scanner system provides a laser-induced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides. The Agilent SureScan Scanner provides a precise and efficient microarray scanner system for many research applications. Designed for research using microarrays, the scanner system provides a laser-induced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides.The microarray instrumentation measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays. The system also enhances productivity with continuous loading from a 24-slide cassette and automatic loading of data files into Feature Extraction Software. It also features built-in ozone protection to minimize signal degradation. 

The Mantis is a medium throughput liquid handler with extremely low dead volume. The liquid handler uses either conventional pipette tips or reagents dispensed from 50 or 15ml Falcon tubes (for continous flow/bulk dispense). The Mantis is ideal for single well dispense of precious reagents. Fully programmable with a simple, yet powerful GUI. Examples of automated protocols using the Mantis within the core. NGS Library Prep (RNA-Seq), Magnetic Bead Dispensing, PCR Master Mix, Precious Reagent Dispensing, Cell Dispensing, PCR Setup.

The Spinnaker robot arm works in conjunction with the two HCS imaging systems and the cytomat 5C hotelling incubator, to provide scheduled autonomous plate imaging capabilities. Book to reserve your inventory position in the cytomat 5C.

Easy-to-use liquid handler that allows for exchange of liquid in 96-well or 384-well formats for changing media, adding compounds, setting up qPCR plates, making plate dilutions, washing plates, fixing plates, staining plates, etc.

The FDSS700ex is an advanced high throughput screening-dispense and imaging sytem for cell-based assays. The instrument optically detects intracellular reaction and biological signal transmissions to discover new lead compounds, which could be candidates for new drugs. Using adherent, suspension or primary cells the FDSS700 is capable of detecting intracellular Ca2+ ion, membrane potential, ion channel. 

The Maestro multielectrode array technology (Axion Biosystems) enables analysis of neural networks and electroactive tissue at unprecedented throughput and scale. This system is ideal for large-scale cellular analysis in drug screening applications and for phenotyping human neurons.

3D/4D visualization and analysis for cell based segmentation, automated tracking, detection, interactive lineage trees and statistical tests (for customization in Matlab, Java or Python).

The base of this system is a standard confocal microscope suitable. Also available at the system is the Leica TCS SP8 STED ONE with white-light laser, for super-resolution STED imaging. 

Equipped with an incubator unit and automated stage, this system allows long term imaging of living samples.

Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence.  

This instrument has a MaiTai tuneable IR laser and 2 channel GaAsP non-descanned detector for multiphoton imaging. Laser lines include 453, 488, 514, 561, 594 and 633.

The MACSQuant VYB is a versatile 3 laser, 10-parameter flow cytometer that has enhanced capabilities for detection of fluorescent protein reporters. It is a fully operated instrument capable of running multi-tube, 96 and 384 plates. 

This instrument is capable of measuring 15 parameters, providing higher flexibility in experiment design for sorting. It is equipped with a 561 and 449 Laser line permitting detecting of Mcherry and cyan protein. This instrument provide all the great features we come to expect with the FACSAria sorters such as customized speed, multi population, variety of collection vessels and index sorting.

This Aria is located in CLS building room 15037. It is inside a self-contain hood allowing for sorting of Primary Human in a complete sterile environment. The CLS-Aria is a bench-top high-speed sorter with fixed- alignment cuvette flow cell for high fluorescence sensitivity.

This Aria can be operated by our staff or users who have been trained and certified by our facility. It is bench-top high-speed sorter with fixed- alignment cuvette flow cell for high fluorescence sensitivity.

Fluorescence detection for quantification using Picogreen or Ribogreen.