Boston Children's Core Equipments

This item was funded in ECRAC cycle 14 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 14 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 12 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 12 and has not yet been purchased. Please contact the core for more information.

This piece of equipment was funded in ECRAC cycle 11 and has not yet been purchased. Please contact the core for more information.

This piece of equipment was funded in ECRAC cycle 11 and has not yet been purchased. Please contact the core for more information.

This piece of equipment was funded in ECRAC cycle 11 and has not yet been purchased. Please contact the core for more information.

This piece of equipment was funded by ECRAC in cycle 1. Please contact the core for more information.

The CFX Connect Real-Time PCR Detection System offers two-target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch Real-Time PCR Detection System. The system incorporates innovative optical technologies with powerful software to provide maximal reliability and efficiency for all your real-time PCR needs.

Harvest different stages of mouse embryos and surgical transfer of manipulated embryos.

Upgraded confocal instrument from LED to Laser, to reduce scan times and increase work flow for high content capture and analysis.

Works with 50 mL conical tubes and 1.5 ml microcentrifuge tubes.

This piece of equipment was funded in ECRAC cycle 6 and has not yet been purchased. Please contact the core for more information.

The Countess™ II FL Automated Cell Counter is a fully automated, 3-channel cell counter and assay platform that uses EVOS™ light cube technology, state-of-the-art optics, and image analysis algorithms to analyze fluorescently labeled cells or trypan blue stained samples in suspension.

This electrophysiology rig with light microscope is capable of single-cell (current-clamp, patch-clamp) and extracellular (local field potential) recordings. Additionally, this rig has two headstages, enabling dual channel recordings, and a temperature control system, allowing for physiological temperatures to be maintained during experiments. The Olympus light microscope with differential interference contrast (DIC) allows optimal visualization of specimens to confirm electrode placement. Lastly, the Lumenera camera allows for images and continuous video (30 fps at full resolution of 1280 x 1024) for documentation during recordings.

This piece of equipment was funded in ECRAC cycle 9 and has not yet been purchased. Please contact the core for more information.

This piece of equipment was funded in ECRAC cycle 9 and has not yet been purchased. Please contact the core for more information.

This piece of equipment was funded in ECRAC cycle 9 and has not yet been purchased. Please contact the core for more information.

This item was funded in ECRAC cycle 10 and has not yet been purchased. Please contact the core for more information.

Dual-energy CT

The Zetaview small particle analyzer employs Nanoparticle Tracking Analysis (NTA) which relates the rate of Brownian motion of the particles in liquid suspension to particle size. , Enders 1052

Used to assess gene expression and perform genotyping, copy number determination, microRNA analyses, and pathogen detection. The system is equipped with interchangeable blocks that are controlled with a Dell Desktop computer containing QuantStudio 6 and 7 Software as well as Primer Express Software. The 384-well format increases throughput and reduces reagent usage; the 96-well Fast block reduces real-time PCR runs from 2 hours to 40 minutes, which will allow 3 times as many experiments to be run each day; and blocks have three temperature zones. Both SYBR Green and TaqMan reactions can be run, with 5 coupled excitation and emission filter channels.

The laboratory is equipped with a VisualSonics Vevo 2100 small animal ultrasound system. This is a high-resolution instrument (30 microns) that can also image at high speed (up to 1000 frames per second). It is capable of imaging in 3D mode as well as M mode, and can also provide pulsed wave Doppler imaging. The device is equipped with three transducers (24, 38 and 55 MHz). The table is heated and includes sensor pads for monitoring the animal during examination. The system is routinely used for cardiac imaging in small animals, and can also be used for tumor vascular imaging with microbubbles.

CO2 and Temp control for live cell monitoring on Keyence epifluorescent scope

Fully automated analysis platform for Nanostring’s nCounter technology, which allows for molecular quantification of a diverse range of DNA, RNA and protein targets through the use of fluorescent probes. Capable of multiplex analysis of up to 800 targets.

Fully automated electrophoresis device for RNA and DNA sample quality assessment. Capable of assessing intactness of extracted genomic DNA or RNA, as well as size and concentration of either nucleic acid. Especially useful for Next-Gen Sequencing quality control, such as library-prep QC.

Capillary electrophoresis system capable of Sanger Sequencing analysis (for NGS confirmation, indel detection, SNP detection, genome editing verification, microbial identification, etc…) and fragment analysis (ie microsatellite analysis).

Inverted dual objective Lightsheet microscope system allows rapid 3-dimensional live and fixed imaging of cells, tissues and organoids. The system is equipped with piezo-controlled dual objectives (20x air, or 40x water), four solid state lasers with AOTF control, X-Y-Z piezo driven motorized stage, specialized chambers, computer and high-resolution monitor. This system is additionally configured for live inverted ratio epifluorescence imaging with a DG4 high-speed switching xenon light source and digital CMOS camera.

This cell-sorting technology allows researchers to simultaneously isolate two cell populations into separate tubes or deposit single or multiple target cells into 96- or 384-well plates.

Resonant scanning confocal microscope equipped with 4 laser lines (405, 488, 514, and 633 nm), hybrid galvo and fast resonant scanning capabilities, ultra-sensitive GaAsP detectors with full spectral imaging, motorized XYZ stage, air and silicone oil objectives, up to 100X, and advanced image analysis with the Olympus CellSens software

Automated imaging system with filter cubes for DAPI, Alexa 488, Alexa 568 and Alexa 647 as well as transmitted light. It can accept slides, multiwell plates and 35mm dishes, has incubation capabilities including CO2 and software for automated capture, image processing and analysis.

Spinning Disk Confocal microscope with 3 laser lines (488, 568, 640), LED epifluorescence and halogen brightfield light sources, temperature controlled enclosure, motorized XY stage, piezo-controlled Z-stage, enabled for confocal, epifluorescence and brightfield fixed and live cell imaging

Multiphoton imaging of fixed or live samples; specialization in deep tissue and Intravital Imaging of live mice

Cell Sorting

Prospective motion correction of MRI images

Amnis ImageStream MarkX

Cell Sorter

Flow Cytometry Analyzer

The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.

The laboratory is equipped with a VisualSonics Vevo 2100 small animal ultrasound system. This is a high-resolution instrument (30 microns) that can also image at high speed (up to 100 frames per second). It is capable of imaging in 3D mode as well as M mode, and can also provide pulsed wave Doppler imaging. The device is equipped with two transducers (25 and 40 MHz) and the Vevo Integrated Rail System, which facilitates alignment of the mouse and the probe as well as image-guided injection, when necessary. The table is heated and includes sensor pads for monitoring the animal during examination. The system is routinely used for cardiac imaging in small animals.

"The Siemens Focus 120 MicroPET is a high-resolution, small animal PET scanner that combines high spatial resolution (<1.3 mm) and high sensitivity (>7%) with a bore size (12 cm diameter and 7.6 axial length) that is optimized for imaging mice and rats. Among commercially available radioactive agents, we are routinely imaging with 18F 2-fluoro-2-deoxy-D-glucose (FDG) and 18F sodium fluoride. We have also carried out some microPET studies with 18F fluorothymidine (FLT) provided by the Division of Nuclear Medicine at Massachusetts General Hospital; we have validated the synthesis of 18F FHBG and have carried out preliminary studies with this tracer."

Threshold heat preference

The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.

The nCounter® SPRINT Profiler makes access to NanoString’s molecular barcoding technology more affordable than ever before for RNA, DNA, and protein profiling applications. Digitally examine multiple pathways in a single tube in an extraction-free workflow. Perform your own data analysis using the  nSolver™ Analysis Software. Compatible with difficult sample types, including FFPE tissue. Highly multiplexed analysis of basic cancer biology and pathway deregulation activity. 

Lecia/Zeiss

Microdialysis

Microendoscope fitted with fiber-coupled laser and LED light sources that is used in conjunction with implantable gradient-index (GRIN) lenses

Conscious, unrestrained ECG

Seizure susceptibility, epileptiform abnormalities, circadian disturbances, actigraphy, auditory evoked response potentials; continuous monitoring available for up to 4 months

Attention/working memory/flexibility

The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.

Short term working memory

Communications

Response to Stress

Emotional response to stimulus/condition

Anxiety/Social Recognition

Anxiety/Cognition

Motor Strength

Forced Activity

Sensorimotor Gating

Visual acuity (brain stem reflex)

Voluntary Activity

This Fortessa is an air-cooled four-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 12 colors + 2 Scatters. Hydrodynamically focused sample is interrogated within a quartz cuvette flow cell. Sample Aspiration rates at 12ul, 35ul, 60ul per minute.

This LSRFortessa is an air-cooled five-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 15 colors + 2 Scatters. They are compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates.

The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.

The FACSAria cell sorter provides customized speed sorting. This instrument is bl2 certified and equipped with an Aerosol Management option for operator protection.  Well designed for your research needs, the FACSAria allows simultaneous sorting 4 populations.  Collection can be done in tubes or Plate.  Index sorting now provides the option of match single cell profile with Fluorescence Intensity. 

CGH & CGH+SNP Microarray Scanner. The Agilent SureScan Scanner provides a precise and efficient microarray scanner system for many research applications. Designed for research using microarrays, the scanner system provides a laser-induced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides. The Agilent SureScan Scanner provides a precise and efficient microarray scanner system for many research applications. Designed for research using microarrays, the scanner system provides a laser-induced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides.The microarray instrumentation measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays. The system also enhances productivity with continuous loading from a 24-slide cassette and automatic loading of data files into Feature Extraction Software. It also features built-in ozone protection to minimize signal degradation. 

Housed in a sterile BL2 cabinet the combi takes advantage of a peristaltic pump to dispense cells into 96 and 384 cell culture plates. Dead volume if around 3-4ml with a dispense taking typically 10-12 seconds. Using this system minimizes varability in dispense volume and reduction in time to dispense multiple plates by hand.

The Agilent Bravo is a versatile liquid handling platform capable of performing multiple tasks controlled from a simple graphical user interface. ADSF lab staff will assist investigators in the programming of custom protocols to facilitate reproducible pipetting for all experiments and assays. The 96-well microtip bravo head provides accurate liquid handling from 0.3-70µl. The Benchcel 4r microplate handler is a combination of automated microplate handling, and storage, that delivers speed and precision from the 4 intergrated microplate stacks and Plateloc microplate sealer. Intergration of the Bravo, Benchcel and Plateloc gives the ADSF a flexible system that can accommodate a wide variety of liquid handling tasks.

The ImageXpress® Micro Confocal High-Content Imaging System is a high content imaging system with confocality giving you the ultimate combination of speed, sensitivity and flexibility. High-resolution dual disk configuration with 60 micron confocal pinhole, 42 micron pinhole confocal and widefield modes. The Environmental Control Option for the ImageXpress Micro Confocal High-Content Imaging System can be configured to mimic conditions within a laboratory incubator (temperature control, gas mixture and humidity) to keep cells alive for long periods of time, making high-content screening kinetic and time lapse experiments a reality. During assay development, the Fluidics option facilitates compound addition and cell washing to characterize potency or to determine incubation times for higher throughput fixed time point assays.

The IncuCyte S3 is an industry leading screening platform for population based high-content analysis (HCA) that adds a new dimension of being able to image the same culture over time. The instrument fits conveniently into a standard CO2 incubator requiring no special adaptation, whilst accommodating up to 6 pieces of labware simultaneously (any plate type that conforms to the ANSI/Society for Lab Automation and Screening (SLAS) standard). The system possesses a fully automated, hands-free operation for periods exceeding 25 days, with automated imaging quality not deviating over these extended periods of time. This enables the end user to establish analysis of long-term cell cultures in vitro, a feature that is lacking in many competing platforms. 

The ArrayScan XTI (ThermoFisher Scientific) features a temperature- and CO2-controlled live cell chamber to assay cell biology over several days. An integrated liquid handler administers compounds well by well, capturing real-time changes in fluorescence with speeds up to 10 Hz with an enhanced. CCD camera and seven-color LED light source, using 6-well to 384-well labware. The instrument is ideally suited for automated image analysis of many cell types, particularly heterogeneous cell preparations that may require detec- tion of co-expressed markers to identify subsets of cells.

(available for use with the Olympus MPE-RS or the Zeiss 710)

An ‘all-in-one’ instrument for tissue clearing. This system is able to process, and clarify a whole adult mouse brain in under 48 hours, allowing the sample to then be immunolabelled (IF/ICC, endogenous expression) and imaged downstream.

System capable of in vivo imaging in anesthesized and awake behaving mice and rats. Custom rig set-up available.

Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence.

Ideal for dissections this microscope uses a variable LED transmitted light base for even illumination of specimens, supplemental fibre optic illuminators, and a rotatable nosepiece with a 0.3 x, 0.5 x and 1 x objectives with programmable motorized zoom. Video capable for protocol development.

This upright confocal is particularly suitable for combinations of GFP/YFP with the new generation of fluorescent proteins (e.g. mOrange/mCherry), and is fitted with a manual stage, facilitating easy location of samples/regions of interest.

The ADVIA is a hematology analyzer for measure blood count. It provides the flexibility of measuring multiple diff species and complete blood count with differential and reticulocyte count. 

MACSVYB is an air-cooled three-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 8 colors + 2 Scatters It is equipped with high throughput sampler that provides rapid, fully automated sample acquisition. They are compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates

This Aria is located in CLS building room 15037. It is inside a self-contain hood allowing for sorting of Primary Human in a complete sterile environment. The CLS-Aria is a bench-top high-speed sorter with fixed- alignment cuvette flow cell for high fluorescence sensitivity.

The 2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of biomolecules. The 2100 Bioanalyzer instrument, together with the 2100 Expert Software and Bioanalyzer assays, provide highly precise analytical evaluation of various samples types in many workflows, including next generation sequencing (NGS), gene expression, biopharmaceutical, and gene editing research. Digital data is provided in a timely manner and delivers objective assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for an accurate result, and the data may be exported in a many different formats for ease-of-use.