Equipment Name | Make/Model | Functionality | Core Facility | Location | Equipment URL |
---|---|---|---|---|---|
Dissecting Microscopes | Leica/Zeiss | Animal Behavior & Physiology Core | Karp 04002/17 | ||
Thermal Place Preference | Bioseb |
Threshold heat preference.
|
Animal Behavior & Physiology Core | Karp 04016 | Thermal Place Preference |
Sleep Deprivation/Fragmentation | Pinnacle Technology |
Sleep Deprivation or fragmentation chambers.
|
Animal Behavior & Physiology Core | Sleep Deprivation/Fragmentation | |
PalmReader | BlackBox Bio |
Machine learning analysis of bottom-up imaging of rodent behavior.
|
Animal Behavior & Physiology Core | BlackBox Bio PalmReader | |
ABI 3730 DNA Analyzers | Thermo Fisher/SeqStudio |
The 48-capillary 3730 DNA Analyzer is the Gold Standard in medium-to-high throughput genetic analysis. Use the 3730 DNA Analyzer for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample. Two are available.
|
Molecular Genetics Core | CLS 16030.21 | ABI 3730 DNA Analyzers |
QIAGEN Automated Nucleic Acid Extraction | QIAGEN/QIAcube |
For fully automated nucleic acid extraction with QIAGEN spin-column kits.
Automates over 80 QIAGEN kits with over 3000 protocols. Allows automated DNA, RNA and protein sample processing. Eliminates manual processing steps. Generates standardized results.
|
Molecular Genetics Core | CLS 16030.23 | QIAGEN Automated Nucleic Acid Extraction |
HPLC | Shimadzu |
Hemoglobin analysis for sickle cell patients
|
TransLab | EN 428 | HPLC |
CliniMACS® Plus | Milteny biotec |
Large scale magnetic cell selection
|
TransLab | EN 423 | CliniMACS® Plus |
SpectraMax i3 | Molecular Devices |
Measures absorbance, fluorescence, and luminescence
|
TransLab | EN 428 | SpectraMax i3 |
Cedex bioanalyzer | Roche |
Cell culture bioprocess analyzer (glucose, lactate etc)
|
TransLab | EN 210 | Cedex bioanalyzer |
QuantStudio™5 Real time PCR | Life Technologies |
Molecular monitoring of patients on gene therapy trials
|
TransLab | EN 447 | QuantStudio™5 Real time PCR |
Droplet Digital™ PCR | BioRad |
Molecular monitoring of patients on gene therapy trials
|
TransLab | EN 428 | Droplet Digital™ PCR |
BD FACSMelody™ | Becton Dickinson |
Cell cytometer, patient monitoring
|
TransLab | EN 447 | BD FACSMelody™ |
MACSQuant® TYTO Cell Sorter | Milteny biotec |
Cell sorting for therapy applications
|
TransLab | EN 447 | MACSQuant® TYTO Cell Sorter |
BD FACSLyric™ | Becton Dickinson |
Flow cytometer, patient monitoring
|
TransLab | EN 447 | BD FACSLyric™ |
Luminex MAGPIX® | Luminex |
Multiplexing analytes
|
TransLab | EN 428 | Luminex MAGPIX® |
STEMvision™ | Stem Cell technologies |
Hematopoietic colony counter
|
TransLab | EN 210 | STEMvision™ |
6-Laser Symphony (Sorter) | BDFACSymphony S6 |
The Symphony is a 6-laser flow cytometry with the flexibility to sort up to 6 ways or into plates or slides. This cytometer helps to isolate subpopulations and can measure 20 or more colors.
|
Flow Cytometry Core | CLS15037 | BDFACSymphony S6 |
Cytek Aurora (Analyzer) | Cytek Aurora |
The Cytek Aurora is a 5-laser flow cytometer equipped with 64 fluorescence channels, providing comprehensive spectrum cytometry capabilities. Additionally, it supports the utilization of up to 40 colors with emission spectra in proximity, eliminating the need for filter changes.
|
Flow Cytometry Core | Karp Rm 8018 | Cytek Aurora (Analyzer) |
IMARIS 10 Image Analysis Software | Oxford Instruments |
3D/4D visualization and analysis for cell based segmentation, automated tracking, detection, interactive lineage trees and statistical tests (for customization in Matlab, Java or Python)
|
Cellular Imaging Core | CLS13035 | IMARIS 10 Image Analysis Software |
LS7 Lightsheet Microscope | Zeiss |
A lightsheet microscope enables full 3D volumetric imaging of transparent samples, both live and fixed. Particular sample preparation (eg. tissue clearing) is required.
|
Cellular Imaging Core | CLS13035 | Zeiss LS7 Lightsheet Microscope |
Tokai Hit Stage Top Environmental Chamber | STR |
CO2 and Temp control for live cell monitoring on Keyence epifluorescent scope
|
Cell Function and Imaging Core | Enders 1313 | Tokai Hit Stage Top Environmental Chamber |
Sh800S Sorter | Sony |
The optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection. Provides the highest level of automation available in any cell sorter, allowing researcher set-up, alignment, calibration and monitoring with a push of a button.
|
Cell Function and Imaging Core | Enders 1300 | Sony SH800S Sorter |
10x Genomics Chromium | 10x Genomics |
Generates individually barcoded sequencing libraries for hundreds of thousands of nanoliter volume oil droplets using up to 1.7 million different barcodes
|
Cell Function and Imaging Core | Enders 625 | Chromium |
Vizgen MERFISH | Vizgen |
MERFISH is an automated, spatially resolved single-cell transcriptome profiling technology. MERFISH combines the power of single-cell transcriptomics with spatial biology by directly visualizing and counting up to a billion RNA transcripts from 100’s to 10,000 genes across 1cm2 tissue slices with single-cell resolution
|
Cell Function and Imaging Core | Enders 1300 | Vizgen MERFISH |
Teledyne Photometrics IRIS 15 sCMOS camera | Photometric IRIS 15 camera |
Microscope Camera
|
Cell Function and Imaging Core | Enders 603 | Teledyne Photometrics IRIS 15 sCMOS camera |
Special Optics/ASI cleared tissue multi-immersion objectives | Special optics cleared tissue objective 0.4NA |
Image tissue in cleared media
|
Cell Function and Imaging Core | Enders 613 | Special Optics/ASI cleared tissue multi-immersion objectives |
Okolab Stagetop Microscope Incubation System | Okolab DiSPIM |
Provide incubation to DiSPIM microscope
|
Cell Function and Imaging Core | Enders 613 | Okolab Stagetop Microscope Incubation System |
Zeiss LSM 980 w/ Airyscan 2 | Zeiss LSM 980 / Axio Observer 7 |
Laser scanning confocal microscope with Airyscan 2 detector array.
|
IDDRC Cellular Imaging Core Facility | Center for Life Sciences: RM 13035 | Zeiss LSM 980 w/ Airyscan 2 |
Class II bio-safety level laminar flow hood with UV light option | Thermo Scientific™/ Catalog number: 1375 |
Mammalian cell/tissue culture
|
Viral Core | CL13055 | Class II bio-safety level laminar flow hood with UV light option |
VTi 50.1 Vertical Rotor Package | Beckman Coulter/ Product No:C78759 |
Optima Series ultracentrifuges
|
Viral Core | CL13065 | VTi 50.1 Vertical Rotor Package |
Midi CO2 Incubator (40 L) equipped with a Digital Mini Rotator | Thermo Scientific™/ Catalog number: 3403 |
Mammalian cell/tissue culture
|
Viral Core | Karp 4038 | Midi CO2 Incubator (40 L) equipped with a Digital Mini Rotator |
SW 32 Ti Swinging-Bucket Rotor Package | Beckman Coulter/ Product No:369694 |
Swinging-Bucket (Ultracentrifugation)
|
Viral Core | CL13067 | SW 32 Ti Swinging-Bucket Rotor Package |
Singulator™ 100 Automated Tissue Dissociation System |
The Singulator 100 system automates the isolation and processing of single cells from tissue samples via microfluidic technology. Maintaining cell viability and integrity, it offers gentle extraction of cells from heterogeneous samples enabling downstream analysis including single cell sequencing and transcriptomics.
|
Molecular Genetics Core | CL16052 | Singulator™ 100 Automated Tissue Dissociation System | |
Illumina NextSeq 1000 | Illumina/NextSeq 500 |
The Illumina NextSeq 1000 uses sequencing-by-synthesis technology to deliver an output range of 10-360Gb at 100M-2.4B reads per run. The NextSeq 1000 uses higher density flow cells for applications including whole genome sequencing, transcriptome analysis, and targeted resequencing. Using the PhiX control library, it provides high-quality and scalable results in as little as 12 hours.
|
Molecular Genetics Core | CL16054 | Illumina NextSeq 1000 |
QIAcube Connect System | QIAcube Connect System FUL-1 |
For fully automated nucleic acid extraction with QIAGEN spin-column kits. Automates over 80 QIAGEN kits with over 3000 protocols. Allows automated DNA, RNA and protein sample processing. Eliminates manual processing steps. Generates standardized results.
|
Molecular Genetics Core | CL16030.21 | QIAcube Connect System |
Covaris R230 Focused-ultrasonicator |
The Covaris R230 Focused-ultrasonicator is designed for precise and controlled shearing of biological samples. It delivers highly reproductible and efficient sample processing to achieve targeted fragment sizes across a wide range of sample types. This system is ideal for several applications including DNA and chromatin shearing, next-generation sequencing library preparation, and chromatin immunoprecipitation (ChIP).
|
Molecular Genetics Core | CL16054 | Covaris R230 Focused-ultrasonicator | |
Fisherbrand Isotemp General Purpose Laboratory Refrigerator |
This two-door Laboratory Refrigerator maintains 2-10C with green refrigerants, adaptative defrost, and temperature alarms.
|
Molecular Genetics Core | CLS16 | Fisherbrand Isotemp General Purpose Laboratory Refrigerator | |
Chromium iX | Chromium iX |
The 10x Genomics Chromium X Series enables single-cell and spatial genomics research via microfluidic technology and parallel sequencing capabilities. For low and high throughput, the Chromium iX handles all single-cell assays and analyzes hundreds of thousands of cells per run.
|
Molecular Genetics Core | CL16054 | Chromium iX |
Bio-Rad C1000 Touch thermal cycler | C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module #1851197 |
The Bio-Rad C1000 Touch thermal cycler is designed for precise polymerase chain reaction (PCR) amplification. It features flexible ramp rates and a wide temperature range to accommodate various PCR protocols and experimental needs.
|
Molecular Genetics Core | CL16030.20 | Bio-Rad C1000 Touch thermal cycler |
Applied Biosystems SeqStudio Flex Series Genetic Analyzer | SEQSTUDIO 24 FLEX |
The SeqStudio Flex Series is a 24 capillary electrophoresis system capable of Sanger Sequencing analysis (for NGS confirmation, indel detection, SNP detection, genome editing verification, microbial identification, etc…) and fragment analysis (ie microsatellite analysis).
|
Molecular Genetics Core | CL16030.19 | Applied Biosystems SeqStudio Flex Series Genetic Analyzer |
Bionano Compute Server | 80014 Bionano Compute Server |
The Bionano Compute Server employs innovative computational power to process and analyze intricate genomic data generated by the Bionano Genomics platform. Leveraging cloud-based infrastructure, these advanced algorithms can process optical genome mapping, structural variant detection, and assembly analysis. Additionally, with its automated analysis pipelines, the Bionano Compute server allows for a high number of large genome samples to be run simultaneously.
|
Molecular Genetics Core | CLS16 | Bionano Compute Server |
Ultimate 3000 BioRS HPLC with MWD and Chromeleon | ThermoFisher Scientific/Ultimate 3000 HPLC |
Ultimate 3000 HPLC with UV/Vis, florescent and electrochemical detectors and Chromeleon analysis software. This is a versatile HPLC system with multiple detectors to quantify a variety of analytes of interest.
|
Animal Behavior and Physiology Core | CLS 13030 | Ultimate 3000 BioRS HPLC with MWD and Chromeleon |
Pinnacle Technologies Inc. Video EEG System | Pinnacle Technologies/Sirenia Sleep Pro |
3-Channel tethered EEG systems with Sirenia Sleep Pro software and IR night vision cameras for sleep scoring.
|
Animal Behavior and Physiology Core | Karp 04013.1 | Pinnacle Technologies Inc. Video EEG System |
NEPA21 in vivo Electroporator | NEPA21 |
In vivo electroporation of mouse embryos
|
Mouse Gene Manipulation Core | EN7.2 | NEPA21 in vivo Electroporator |
Eppendorf micro manipulators 4r, Femotojet 4i injector, CellTram Air - pneumatic microinjector, CellTram Oil hydraulic microinjector | Eppendorf 4r, 4i, CellTram Air, CellTram Oil |
Mouse embryo micromanipulation and microinjection
|
Mouse Gene Manipulation Core | EN7.2 | TransferMan 4r, FemtoJet 4i, CellTram 4m Air and CellTram 4m Oil |
Phenome Technologies Circadian Cabinets and AXIS IP Cameras | 2 Modified (PT2-CCM3-MOD); 1 unmodified (PT2-CCM3) mouse cabinets; AXIS M4216-LV IP cameras | Animal Behavior and Physiology Core | RB4013.1 | Phenome Technologies Circadian Cabinets AXIS IP Cameras | |
Phenome Technologies Circadian Cabinets with Actimetrics ClockLab Chamber Control Software | 3 Modified (PT2-CCM3-MOD); 2 unmodified (PT2-CCM3) mouse cabinets; Clocklab Chamber Control Software (AM1-CC02) | Animal Behavior and Physiology Core | RB4013.1 | Phenome Technologies Circadian Cabinets Actimetrics ClockLab Chamber Control Software | |
GPU work station for cryo-EM data processing |
This item was funded in ECRAC cycle 17 and has not yet been purchased. Please contact the core for more information.
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PCMM Microscopy Core | GPU work station for cryo-EM data processing | ||
Class II bio-safety level laminar flow hood with UV light option |
This item was funded in ECRAC cycle 17 and has not yet been purchased. Please contact the core for more information.
|
Viral Core | Class II bio-safety level laminar flow hood with UV light option | ||
Singulator™ 100 Automated Tissue Dissociation System |
This item was funded in ECRAC cycle 17 and has not yet been purchased. Please contact the core for more information.
|
Molecular Genetics Core | Singulator™ 100 Automated Tissue Dissociation System | ||
Cytek Aurora FACS |
This item was funded in ECRAC cycle 17 and has not yet been purchased. Please contact the core for more information.
|
PCMM | Cytek Aurora FACS | ||
Perkin Elmer Opera Phenix Plus High-Content Screening System | Revvity (former Perkin Elmer) |
A spinning disk confocal microscope is used for high-throughput, high-content assays, phenotypic screening, assays using complex disease models, such as live cells, primary cells, and microtissues, and fast-response assays, such as Ca2+ flux. It is equipped with a liquid handling environmental chamber, 5 lasers and 4 cameras, a full set of objectives, 5x air, 10x air, 20x air, 20x water, 40x water, and 63x water objectives.
|
Human Neuron Core | CLS 12 121 | Perkin Elmer Opera Phenix Plus High-Content Screening System |
Andor BC43 Benchtop Confocal Microscope |
Benchtop spinning disk confocal with widefield capabilities. User-friendly software and speedy acquisition. Laser choices are 405nm, 488nm, 561nm and 631nm.
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Cell Function and Imaging Core | Enders 613 | Andor BC43 Benchtop Confocal Microscope | |
NEPA21 |
This item was funded in ECRAC cycle 16 and has not yet been purchased. Please contact the core for more information.
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Mouse Gene Manipulation Core | NEPA21 | ||
Covaris R230 Focused-ultrasonicator |
This item was funded in ECRAC cycle 16 and has not yet been purchased. Please contact the core for more information.
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Molecular Genetics Core | Covaris R230 Focused-ultrasonicator | ||
MaxCyte ExPERT GTx scalable transfection system | Maxcyte |
Scalable electroporator primarily used for gene editing (clinical)
|
TransLab | EN 206 | MaxCyte ExPERT GTx scalable transfection system |
AutoSampler for ImageStreamX Mark II Imaging Flow Cytometer |
This item was funded in ECRAC cycle 16 and has not yet been purchased. Please contact the core for more information.
|
PCMM Flow and Imaging Cytometry Resource | AutoSampler for ImageStreamX Mark II Imaging Flow Cytometer | ||
VTi 50.1 Vertical Rotor Package |
This item was funded in ECRAC cycle 15 and has not yet been purchased. Please contact the core for more information.
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Viral Core | VTi 50.1 Vertical Rotor Package | ||
BD FACSymphony™ A5 Cell Analyzer |
This item was funded in ECRAC cycle 15 and has not yet been purchased. Please contact the core for more information.
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Cell Function and Imaging Core | BD FACSymphony™ A5 Cell Analyzer | ||
Fisherbrand Isotemp General Purpose Laboratory Refrigerator |
This item was funded in ECRAC cycle 15 and has not yet been purchased. Please contact the core for more information.
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Molecular Genetics Core | Fisherbrand Isotemp General Purpose Laboratory Refrigerator | ||
Chromium iX |
This item was funded in ECRAC cycle 15 and has not yet been purchased. Please contact the core for more information.
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Molecular Genetics Core | Chromium iX | ||
Lenovo HPC nodes (SIDX00KBDU) |
This item was funded in ECRAC cycle 14 and has not yet been purchased. Please contact the core for more information.
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Research Computing | Lenovo HPC nodes (SIDX00KBDU) | ||
Leica Thunder Imaging System | Leica Thunder |
High end, automated widefield microscope with full incubation and phase transmission imaging. Excellent characteristics for a widefield with a massive amount of excitation bands including CY7 and Alexa 750 support. Has built in computational clearing methods as well. Absolute breeze at whole slide acquisitions and long-term live experiments.
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Cell Function and Imaging Core | Enders 403 | Leica Thunder Imaging System |
Circadian controlled housing boxes and ip Cameras |
This item was funded in ECRAC cycle 14 and has not yet been purchased. Please contact the core for more information.
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Animal Behavior and Physiology Core | Circadian controlled housing boxes and ip Cameras | ||
Bio-Rad C1000 Touch thermal cycler |
This item was funded in ECRAC cycle 14 and has not yet been purchased. Please contact the core for more information.
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Molecular Genetics Core | Bio-Rad C1000 Touch thermal cycler | ||
Eppendorf micro manipulators 4r, Femotojet 4i injector, CellTram Air - pneumatic microinjector, CellTram Oil hydraulicmicroinjector |
This item was funded in ECRAC cycle 14 and has not yet been purchased. Please contact the core for more information.
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Mouse Gene Manipulation Core | Eppendorf micro manipulators 4r, Femotojet 4i injector, CellTram Air - pneumatic microinjector, CellTram Oil hydraulicmicroinjector | ||
Rodent barostat and associated equipment (amplifier, analyzer, and software) necessary for measuring visceral sensitivity in mice. |
This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.
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Animal Behavior and Physiology Core | Rodent barostat and associated equipment (amplifier, analyzer, and software) necessary for measuring visceral sensitivity in mice. | ||
Nanopore GridION Mk1 Sequencing System |
This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.
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Molecular Genetics Core | Nanopore GridION Mk1 Sequencing System | ||
Midi CO2 Incubator (40 L) equipped with a Digital Mini Rotator |
This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.
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Viral Core | Midi CO2 Incubator (40 L) equipped with a Digital Mini Rotator | ||
Applied Biosystems SeqStudio Flex Series Genetic Analyzer | A53631 |
This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.
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Molecular Genetics Core | Applied Biosystems SeqStudio Flex Series Genetic Analyzer | |
JASCO FP-8350 Spectrofluorometer | Jasco FP-8350 |
Measure Fluorescence Spectra
|
Cell Function and Imaging Core | Enders 611 | JASCO FP-8350 Spectrofluorometer |
Actimetrics Circadian Cabinets with ClockLab Chamber Control Software |
This item was funded in ECRAC cycle 13 and has not yet been purchased. Please contact the core for more information.
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Animal Behavior and Physiology Core | Actimetrics Circadian Cabinets with ClockLab Chamber Control Software | ||
Zeiss LSM 980 Airyscan2 microscope | Zeiss |
A laser scanning confocal microscope enables full 3D imaging of both live and fixed samples. The airyscan detector provides faster image acquisition and higher resolution than typical confocal imaging.
|
Cellular Imaging Core | CLS13035 | Zeiss LSM 980 Airyscan2 microscope |
Columbus Instruments 2 lane metabolic treadmill | 1012M-2 Modular Enclosed Metabolic Treadmill for Mice, 2 Lanes w/ Shock |
This item was funded in ECRAC cycle 12 and has not yet been purchased. Please contact the core for more information. |
Animal Behavior and Physiology Core | Columbus Instruments 2 lane metabolic treadmill | |
Bionano Compute Server |
This item was funded in ECRAC cycle 12 and has not yet been purchased. Please contact the core for more information.
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Molecular Genetics Core | Bionano Compute Server | ||
TECAN SPARK Multi-mode Plate Reader | Tecan Spark |
: Multiwell plate reader, fluorescence transmission
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Cell Function and Imaging Core | Enders 603 | TECAN SPARK Multi-mode Plate Reader |
Ultimate 3000 BioRS HPLC with MWD and Chromeleon |
This item was funded in ECRAC cycle 12 and has not yet been purchased. Please contact the core for more information.
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Animal Behavior and Physiology Core | Ultimate 3000 BioRS HPLC with MWD and Chromeleon | ||
AxioImager Slide-scanning Microscope | Zeiss |
Able to obtain widefield images for fluorescent and/or colorimetric/histology stains, complete with a slide-scanning stage for up to 8 slides.
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Cellular Imaging Core | CLS 13035 | AxioImager Slide-scanning Microscope |
Luna-FL Automated Fluorescent and Brightfield Cell Counter |
The LUNA-FL Cell Counter fluorescence optics and image analysis for precise cell counting and viability analysis. Using two fluorescence channels and automated analysis, it simultaneously detects multiple stains for live and dead cells.
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CL16052 |
Luna-FL Automated Fluorescent and Brightfield Cell Counter |
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SW 32 Ti Swinging-Bucket Rotor Package |
This piece of equipment was funded in ECRAC cycle 11 and has not yet been purchased. Please contact the core for more information.
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SW 32 Ti Swinging-Bucket Rotor Package |
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GPU computing node |
Hardware for cloud computing
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Research Computing BCH facility (O2 cluster) |
CFIC cluster computation node |
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Pinnacle Technologies Inc. Video EEG System |
This piece of equipment was funded in ECRAC cycle 11 and has not yet been purchased. Please contact the core for more information.
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Pinnacle Technologies Inc. Video EEG System |
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FPLC Machine |
This piece of equipment was funded by ECRAC in cycle 1. Please contact the core for more information.
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Automated CFU Counter |
This piece of equipment was funded by ECRAC in cycle 1. Please contact the core for more information.
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EGI REP Model |
This piece of equipment was funded by ECRAC in cycle 1. Please contact the core for more information.
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Bio-Rad CFX Connect Real-Time PCR Detection System | Bio-Rad CFX Connect Optics module |
The CFX Connect Real-Time PCR Detection System offers two-target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch Real-Time PCR Detection System. The system incorporates innovative optical technologies with powerful software to provide maximal reliability and efficiency for all your real-time PCR needs.
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CL 13060.13 | ||
Sorvall LYNX 4000; Superspeed Floor Centrifuge and four compatible rotors |
This piece of equipment was funded in ECRAC cycle 3. Please contact the core for more information.
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Leica M80 Dissecting Microscopes | Leica/M80 |
Harvest different stages of mouse embryos and surgical transfer of manipulated embryos.
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ARCH EN 007.2 | ||
Data Sciences International Ponemah EEG Acquisition System |
Please contact the core for more information. |
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IXM-C 1000mW laser upgrade |
Upgraded confocal instrument from LED to Laser, to reduce scan times and increase work flow for high content capture and analysis.
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CLS 12121 | |||
Nanostring n-counter system |
The NanoString nCounter Analysis System employs a unique digital barcoding technology to quantitatively measure gene and protein expression in biological samples with high precision and sensitivity. This platform enables simultaneous analysis of multiple targets without the need for additional amplification steps.
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CLS16 | |||
5430R Centrifuge, refrigerated, KEYPAD, with 6x15/50ml ROTOR, 120V | Eppendorf5430R |
Works with 50 mL conical tubes and 1.5 ml microcentrifuge tubes.
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CLS 13060.8, 13060.9, 13060.10 |
5430R Centrifuge, refrigerated, KEYPAD, with 6x15/50ml ROTOR, 120V |
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Celeris ERG system |
Rodent electroretinography
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Animal Behavior & Physiology Core | Karp 4 Room 04004 | Celeris ERG system | |
Okolab Stagetop Microscope Incubation System |
This piece of equipment was funded in ECRAC cycle 6 and has not yet been purchased. Please contact the core for more information.
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Cell Function and Imaging Core | Okolab Stagetop Microscope Incubation System | ||
Special Optics/ASI cleared tissue multi-immersion objectives |
This piece of equipment was funded in ECRAC cycle 6 and has not yet been purchased. Please contact the core for more information.
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Cell Function and Imaging Core | Special Optics/ASI cleared tissue multi-immersion objectives | ||
Countess II FL Automated Cell Counter | AMQAF1000 |
The Countess™ II FL Automated Cell Counter is a fully automated, 3-channel cell counter and assay platform that uses EVOS™ light cube technology, state-of-the-art optics, and image analysis algorithms to analyze fluorescently labeled cells or trypan blue stained samples in suspension.
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Viral Core | CLS 13064 | Countess II FL Automated Cell Counter |
Sartorius IncuCyte S3 Live-Cell Analysis System | Sartorius IncyCyte S3 |
Automated collection, measurement and analysis of culture in situ, for short/long term time lapse experiments.
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Microscopy Core, Program in Cellular and Molecular Medicine | 210 Longwood Ave., Amenise 2 Room 235 | Sartorius IncuCyte S3 Live-Cell Analysis System |
Electrophysiology rig with light microscope |
This electrophysiology rig with light microscope is capable of single-cell (current-clamp, patch-clamp) and extracellular (local field potential) recordings. Additionally, this rig has two headstages, enabling dual channel recordings, and a temperature control system, allowing for physiological temperatures to be maintained during experiments. The Olympus light microscope with differential interference contrast (DIC) allows optimal visualization of specimens to confirm electrode placement. Lastly, the Lumenera camera allows for images and continuous video (30 fps at full resolution of 1280 x 1024) for documentation during recordings.
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Animal Behavior & Physiology Core | CLS14041 | Electrophysiology rig with light microscope | |
Max 4000 Benchtop Orbital Shakers | SHKE4000 |
With a spacious 18 × 18 in. platform for high vessel capacity and precisely monitored temperature and speed, MaxQ™ 4000 Benchtop Orbital Shaker is an ideal equipment for E.coli culture.
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Viral Core | Bay 13060.1 | Max 4000 Benchtop Orbital Shakers |
The Thermo Scientific Savant SpeedVac SPD1030 system |
The Thermo Scientific Savant SpeedVac uses vacuum technology to expedite drying processes and efficiently concentrate and evaporate samples.
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Molecular Genetics Core | CLS16 | ||
Teledyne Photometrics IRIS 15 sCMOS camera |
This piece of equipment was funded in ECRAC cycle 9 and has not yet been purchased. Please contact the core for more information.
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Cell Function and Imaging Core | |||
Zeto EEG headset |
This piece of equipment was funded in ECRAC cycle 9 and has not yet been purchased. Please contact the core for more information.
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TNC Human Neurophysiology Core | Zeto EEG headset | ||
Nikon Eclipse Ti2E inverted microscope | Nikon Eclipse Ti2-E |
Motorized, live-imaging compatible, inverted microscope for automated high content and confocal applications, including calcium imaging and optogenetics.
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Human Neuron Core | CLS12161.1 | Nikon Eclipse Ti2E inverted microscope |
FujiFilm VisualSonics Vevo 3100 Imaging System |
This piece of equipment was funded in ECRAC cycle 9 and has not yet been purchased. Please contact the core for more information.
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Heracell 240i CO2 Incubator | 51032877 |
The Heracell 240i Incubator, which is equipped with three rows of bottle-turning devices for roller bottles, each with independent speed control, is suitable for scaling up mammalian cell culture.
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Viral Core | CLS 13064 | Heracell 240i CO2 Incubator |
Huygens Professional Image Processing Software | Huygens professional |
Deconvolution images |
Cell Function and Imaging Core | Enders 603 stedycon computer | Huygens Professional Image Processing Software |
Echo655 acoustic liquid handler |
Nanoliter dispensing of compounds for drug screening/high throughput screening.
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Human Neuron Core | SGM640 | Echo655 acoustic liquid handler | |
Zeiss LSM980 Airyscan2 Confocal Microscope | This item was funded in ECRAC cycle 10 and has not yet been purchased. Please contact the core for more information. | Cellular Imaging Core | Zeiss LSM980 Airyscan2 Confocal Microscope | ||
Abberior StedyCon Stimulated Depletion (STED) super-resolution microscope | Abberior STEDYCON |
Super resolution microscope capable of 30nm resolution. STED is a very easy and user-friendly technique. Literally just swap secondary labelled for Star Red and or Orange fluorophores, that’s it! Really excels at colocalization experiments as well. Huygens decon available for use.
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Cell Function and Imaging Core | Enders 603 | Abberior StedyCon Stimulated Depletion (STED) super-resolution microscope |
CT Scanners | Siemens Somatom Force |
Dual-energy CT
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Research Imaging Core | Boston | CT Scanners |
Digital Radiography | Philips Digital Diagnostic |
Diagnostic X-ray exams
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Boston, Peabody, Waltham, Weymouth | Digital Radiography | |
Quant Nanoparticle Analyzer | Zetaview |
The Zetaview small particle analyzer employs Nanoparticle Tracking Analysis (NTA) which relates the rate of Brownian motion of the particles in liquid suspension to particle size.
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Cell Function and Imaging Core | Enders 1052 | Quant Nanoparticle Analyzer |
Tape Station 4200 | Agilent |
The Agilent 4200 TapeStation system is an established automated electrophoresis tool for DNA and RNA sample quality control. This tape station does fully automated sample processing which enables the unattended analysis of size, concentration and integrity.
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Cell Function and Imaging Core | Enders 1317 | Agilent 4200 TapeStation |
Real-time PCR System | Applied Biosystems |
Used to assess gene expression and perform genotyping, copy number determination, microRNA analyses, and pathogen detection. The system is equipped with interchangeable blocks that are controlled with a Dell Desktop computer containing QuantStudio 6 and 7 Software as well as Primer Express Software. The 384-well format increases throughput and reduces reagent usage; the 96-well Fast block reduces real-time PCR runs from 2 hours to 40 minutes, which will allow 3 times as many experiments to be run each day; and blocks have three temperature zones. Both SYBR Green and TaqMan reactions can be run, with 5 coupled excitation and emission filter channels. |
Vascular Biology Program | Karp Family Research Laboratories 12.0011.1 Alcove | Real-time PCR System |
Tokai Hit Environmental Chamber for Keyence BZ-X700 | STR |
CO2 and Temp control for live cell monitoring on Keyence epifluorescent scope |
Molecular Genetics Core | ||
Chromium Controller | 10x Genomics/Chromium Controller |
Partitioning device used to isolate single cells from a cell suspension in “Gel Bead-in-Emulsion” droplets, where nucleic acid of interest is extracted and tagged with a cell-specific barcode for downstream Next-Gen Sequencing library prep and sequencing.
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Molecular Genetics Core | CLS 16030.23 | Chromium Controller |
4200 TapeStation | Agilent/4200 TapeStation |
Fully automated electrophoresis device for RNA and DNA sample quality assessment. Capable of assessing intactness of extracted genomic DNA or RNA, as well as size and concentration of either nucleic acid. Especially useful for Next-Gen Sequencing quality control, such as library-prep QC.
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Molecular Genetics Core | CLS 16030.23 | 4200 TapeStation |
NanoDrop 8000 Spectrophotometer | ThermoFisher/NanoDrop 8000 |
Microvolume UV-Vis and Fluorescence spectrophotometer used for nucleic acid and protein quantification and quality assessment. Additionally capable of Microarray fluorescent labeling QC analysis, as well as Pierce 660, Bradford, BCA, Lowry assays. 8000 model has 8 channels improving speed.
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Molecular Genetics Core | CLS 16030.23 | |
SeqStudio Genetic Analyzer | Applied Biosystems/SeqStudio |
Capillary electrophoresis system capable of Sanger Sequencing analysis (for NGS confirmation, indel detection, SNP detection, genome editing verification, microbial identification, etc…) and fragment analysis (ie microsatellite analysis).
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Molecular Genetics Core | CLS 16030.21 |
<a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/sanger-s..."> SeqStudio Genetic Analyzer </a> |
Centrifuge | Eppendorf 5430R |
Works with 50 mL conical tubes and 1.5 ml microcentrifuge tubes
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CLS 13060.8, CLS 13060.9, CLS 13060.10 | Centrifuge | |
DiSPIM Lightsheet microscope | ASI |
Inverted dual objective Lightsheet microscope system allows rapid 3-dimensional live and fixed imaging of cells, tissues and organoids. The system is equipped with piezo-controlled dual objectives (20x air, or 40x water), four solid state lasers with AOTF control, X-Y-Z piezo driven motorized stage, specialized chambers, computer and high-resolution monitor. This system is additionally configured for live inverted ratio epifluorescence imaging with a DG4 high-speed switching xenon light source and digital CMOS camera.
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Cell Function and Imaging Core | Enders 613 | DiSPIM Lightsheet microscope |
Seahorse Xfe 96 Analyzer | Agilent |
Analyzer measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. These rates are key indicators of mitochondrial respiration and glycolysis. They provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
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Cell Function and Imaging Core | Enders 622 | Seahorse Xfe 96 Analyzer |
FACSMelody Cell Sorter | BD Bioscience |
This cell-sorting technology allows researchers to simultaneously isolate two cell populations into separate tubes or deposit single or multiple target cells into 96- or 384-well plates.
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Cell Function and Imaging Core | Enders 613 |
FACSMelody Cell Sorter |
FACSCanrto II | BD Bioscience |
Analysis of cell surface or intracellular proteins or other “markers,” intracellular cytokine production and receptor-mediated endocytosis, sorting, and recycling.
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Cell Function and Imaging Core | Enders 613 | FACSCanrto II |
BD LSRFortess | BD Bioscience |
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Most precise and sensitive digital PCR solution for a wide variety of applications.
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Cell Function and Imaging Core | Enders 613 | BD LSRFortess |
Kinetic Imaging Cytometer (KIC) | Vala |
High content live cell imager capable of electrical or optical stimulation and high-speed acquisition with an analysis package designed for quantifying parameters from individual cells within a well
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Cell Function and Imaging Core | Enders 1250 |
Kinetic Imaging Cytometer (KIC) |
FV300R | Olympus |
Resonant scanning confocal microscope equipped with 4 laser lines (405, 488, 514, and 633 nm), hybrid galvo and fast resonant scanning capabilities, ultra-sensitive GaAsP detectors with full spectral imaging, motorized XYZ stage, air and silicone oil objectives, up to 100X, and advanced image analysis with the Olympus CellSens software
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Cell Function and Imaging Core | Enders 1347 |
FV300R |
BZ-X700 | Keyence |
Epifluorescent, brightfield, and phase imaging, image stitching for capturing images larger than one field, automated multiwell plate image sample, structured illumination, live cell imaging with video rate capture and environmental controls and built-in image analysis functions.
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Cell Function and Imaging Core | Enders 1347 | BZ-X700 |
Cytation 5 | Biotek |
Automated imaging system with filter cubes for DAPI, Alexa 488, Alexa 568 and Alexa 647 as well as transmitted light. It can accept slides, multiwell plates and 35mm dishes, has incubation capabilities including CO2 and software for automated capture, image processing and analysis.
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Cell Function and Imaging Core | Enders 620 | Cytation 5 |
BX41 upright Histology Microscope | Olympus |
Upright microscope with a Pixelink PL-D682CU color CMOS camera for brightfield and epifluorescence imaging.
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Cell Function and Imaging Core | Enders 603 |
BX41 upright Histology Microscope |
Axiovert Spinning Disk Confocal | Zeiss |
Spinning Disk Confocal microscope with 3 laser lines (488, 568, 640), LED epifluorescence and halogen brightfield light sources, temperature controlled enclosure, motorized XY stage, piezo-controlled Z-stage, enabled for confocal, epifluorescence and brightfield fixed and live cell imaging
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Cell Function and Imaging Core | Enders 613 |
Axiovert Spinning Disk Confocal |
LSM 880 | Zeiss |
Laser Scanning Confocal microscope with 6 laser lines (405/458/488/514/561/633nm), Fast Airyscan for near super-resolution confocal imaging, LED epifluorescence and halogen brightfield light sources, temperature controlled enclosure, motorized XY stage, piezo-controlled Z-stage, enabled for confocal, brightfield, and DIC, fixed and live cell imaging.
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Cell Function and Imaging Core | Enders 613 | LSM 800 |
Multiphoton Microscope | Olympus FV1200 MPE Multiphoton Microscope (with BX 61 microscope setup) |
Multiphoton imaging of fixed or live samples; specialization in deep tissue and Intravital Imaging of live mice
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PCMM Microscopy Core | 200 Longwood Ave, WAB Rm247a |
Multiphoton Microscope |
Confocal Microscope | Olympus FV1000 Confocal Microscope (with IX81 microscope setup) |
Confocal imaging of fixed or live samples
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PCMM Microscopy Core | CLS 3rd Floor, Rm 03123 | Confocal Microscope |
Sony MA900 Sorter | MA900 |
Cell Sorting
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hESC Core Facility | RB 5214 | Sony MA900 Sorter |
CV7000 Imager | CV7000 |
High-throughout high-content confocal fluorescence imaging.
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hESC Core Facility | RB05214 |
CV7000 Imager |
Motion tracking system | KinetiCor |
Prospective motion correction of MRI images
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For more information contact Simon Warfield at Simon.Warfield@childrens.harvard.edu |
Boston |
Motion tracking system |
fMRI equipment | NordicNeuroLab fMRI equipment |
functional MRI data collection
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For more information contact Simon Warfield at Simon.Warfield@childrens.harvard.edu |
Boston Waltham |
fMRI equipment |
ImageStream | Amnis ImageStream MarkX |
Amnis ImageStream MarkX
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Flow Cytometry PCMM | ARM 231, 200 Longwood Ave | ImageStream |
BL2 Aria Cell Sorter | BD FACS Aria II |
BL2 Cell Sorter
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Flow Cytometry PCMM | ARM 239, 200 Longwood Ave. | BL2 Aria Cell Sorter |
Stony Cell Sorter | Sony SH800Z |
Cell Sorter
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Flow Cytometry PCMM | WAB 109, 200 Longwood Ave. |
Stony Cell Sorter |
CLSB-Canto Flow Cytometry Analyzer | BD FACS CantoII |
Flow Cytometry Analyzer with Plate Reader
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Flow Cytometry PCMM | CLSB 3034, 3 Blackfan Circle | CLSB-Canto Flow Cytometry Analyzer |
AR-CantoII Flow Cytometry Analyzer | BD FACS Cantoll |
Flow Cytometry Analyzer
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Flow Cytometry PCMM | Arm 239, 200 Longwood Ave |
AR-CantoII Flow Cytometry Analyzer |
Dual inverted selective plane illumination microscope | ASI/DiSPIM |
Light Sheet Microscope, primary niche is rapid low light dose imaging of 3D objects.
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Cellular Imaging Core | Enders 613 | Dual inverted selective plane illumination microscope |
Thermo Fisher Stericult double stacked relative humidity-controlled incubator | Human Neuron Core | Thermo Fisher Stericult double stacked relative humidity-controlled incubator | |||
Neurolucida and Stereo Investigator | Nikon |
Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence. |
Cellular Imaging Core | CLS 12 12145 | Nikon 80i with Neurolucida and Stereo Investigator |
Biorad Droplet Digital PCR System | BIORAD/QX200 with Auto DG |
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Most precise and sensitive digital PCR solution for a wide variety of applications. |
Molecular Genetics | CLS 16 16030.22 | Biorad Droplet Digital PCR System |
Magnetic Resonance |
For more information contact Simon Warfield at Simon.Warfield@childrens.harvard.edu |
Magnetic resonance |
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Von Frey | Stoelting |
Mechanical allodynia threshold
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Animal Behavior & Physiology Core | Karp RM 04009 | Von Frey |
Thermal Gradient | Bioseb |
Threshold heat preference
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Animal Behavior & Physiology Core | Karp RM 04016 | Thermal Gradient |
NextSeq 500 | Illumina/NextSeq 500 |
Provides high quality, high coverage genome with streamlined library preparation using high quality TruSeq library preparation kits. Single, paired-end, and indexed sequencing with accuracy of > 75% of sequenced bases over Q30. Enables researchers to interrogate simple prokaryotic and complex eukaryotic genomes quickly and efficiently.
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Molecular Genetics | CLS 16 16054 | NextSeq 500 |
CFX384 Touch Real-Time PCR Detection System | Biorad/CFX384 |
The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.
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Molecular Genetics | CLS 16 16054 | CFX384 Touch Real-Time PCR Detection System |
CFX 96 and CFX384 Touch Real-Time PCR Detection System | Biorad/CFX96 |
The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.
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Molecular Genetics | CLS 16 16054 | 4CFX 96 and CFX384 Touch Real-Time PCR Detection System |
Bionano Saphyr | Bionano/Saphyr |
The Saphyr System by Bionano Genomics is a whole genome imaging tool for high-speed, high-throughput structural variant detection and analysis with exceptional sensitivity and specificity. Wide range of applications includes: undiagnosed genetic disorders, hematologic malignancies, gene discovery and therapy, cell line stability, solid tumor research, genetic engineering studies, evolutionary biology, and reference genome assembly. Resolve large-scale structural variations missed by next-generation sequencing systems. Detect structural variants with high specificity and sensitivity.
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Molecular Genetics | CLS 16049 | Bionano Saphyr |
nCounter® SPRINT Profiler | Nanostring/Sprint Profiler |
The nCounter® SPRINT Profiler makes access to NanoString’s molecular barcoding technology more affordable than ever before for RNA, DNA, and protein profiling applications. Digitally examine multiple pathways in a single tube in an extraction-free workflow. Perform your own data analysis using the nSolver™ Analysis Software. Compatible with difficult sample types, including FFPE tissue. Highly multiplexed analysis of basic cancer biology and pathway deregulation activity.
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Molecular Genetics Core | CLS 16049 | nCounter® SPRINT Profiler |
Stereotaxic frames | Harvard Apparatus | Animal Behavior & Physiology Core | Karp RM 04002/17 | Stereotaxic Frames | |
Dissecting microscopes | Leica/Zeiss |
Lecia/Zeiss
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Animal Behavior & Physiology Core | Karp RM 04002/17 | Leica/Zeiss |
In vivo microdialysis | BASi Raturn and BAS HoneyComb |
Microdialysis
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Animal Behavior & Physiology Core | In vivo microdialysis | |
Open flow microperfusion | BASi Microperfusion |
Microdialysis
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Animal Behavior & Physiology Core | Karp 04009 | Open flow microperfusion |
In vivo calcium imaging | Neurophotometrics |
Multi-fiber photometry
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Animal Behavior & Physiology Core | Karp 4 Portable | In vivo calcium imaging |
Calcium imaging and Optogenetics | OASIS (Mightex) |
Microendoscope fitted with fiber-coupled laser and LED light sources that is used in conjunction with implantable gradient-index (GRIN) lenses
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Animal Behavior & Physiology Core | Karp 4 Portable | Calcium imaging and Optogenetics |
Whole Body Plethysmography | SCIREQ (EMKA Technologies) |
Breathing measures, apneas
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Animal Behavior & Physiology Core | Karp 04009 | Whole Body Plethysmography |
ECGenie | Mouse Specifics |
Conscious, unrestrained ECG
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Animal Behavior & Physiology Core | Karp 04009 | ECGenie |
TMS with electromyography and/or accelerometry | TMS with electromyography and/or accelerometry |
In vivo cortical stimulation for translational experiments aimed to measure cortical excitability, cortical inhibition, cortical plasticity, motor evoked potentials
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Animal Behavior & Physiology Core | CLS 14 | TMS with electromyography and/or accelerometry |
Subdermal wire electrode EEG | Cadwell | Animal Behavior & Physiology Core | Karp RM 04013 | Subdermal wire electrode EEG | |
Tethered EEG, multichannel | Pinnacle Technology |
Sleep architecture analysis for up to 5 days
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Animal Behavior & Physiology Core | Karp RM 04013 | Tethered EEG, multichannel |
Wireless EEG | DSI (Harvard Bioscience) |
Seizure susceptibility, epileptiform abnormalities, circadian disturbances, actigraphy, auditory evoked response potentials; continuous monitoring available for up to 4 months
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Animal Behavior & Physiology Core | Karp RM 04013 | Wireless EEG |
Hot plate | Bioseb |
Noxious heat sensitivity
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Animal Behavior & Physiology Core | Karp RM 04016 | Hot plate |
Operant Touchscreens | Lafayette Instruments |
Attention/working memory/flexibility
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Animal Behavior & Physiology Core | Karp RM 04015 | Operant Touchscreens |
Morris Water Maze | Ethovision XT (Noldus) |
Spatial short term working memory
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Animal Behavior & Physiology Core | Karp RM 04008 | Morris Water Maze |
Water T Maze | Ethovision XT (Noldus) |
Cognitive flexibility.
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Animal Behavior & Physiology Core | Karp RM 04014 | Water T Maze |
Y Maze | Ethovision XT (Noldus) |
Spatial short term working memory
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Animal Behavior & Physiology Core | Karp RM 04014 | Y Maze |
Novel Object Recognition | Ethovision XT (Noldus) |
Short term working memory
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Animal Behavior & Physiology Core | Karp RM 04014 | Novel Object Recognition |
Contextual Fear Conditioning | Stoelting (ANY-maze) |
Anxiety/Cognition
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Animal Behavior & Physiology Core | Karp RM 04006 | Contextual Fear Conditioning" |
Ultrasonic Vocalizations | Sonotrack, Metris |
Communications
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Animal Behavior & Physiology Core | Karp RM 04016 | BIORAD Droplet Digital PCR System |
Open field/holeboards | Kinder Scientific |
Anxiety Activity
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Animal Behavior & Physiology Core | Karp RM 04016 | Open field/holeboards |
Tail Suspension | Ethovision XT (Noldus) |
Response to Stress
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Animal Behavior & Physiology Core | Karp RM 04016 | Tail Suspension |
Forced Swim | Ethovision XT (Noldus) |
Response to Stress
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Animal Behavior & Physiology Core | Karp RM 04016 | Forced Swim |
Conditioned Place Preference | Ethovision XT (Noldus) |
Emotional response to stimulus/condition
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Animal Behavior & Physiology Core | Karp RM 04014 | Conditioned Place Preference |
Elevated Plus Maze |
Anxiety
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Animal Behavior & Physiology Core | Elevated Plus Maze | ||
3- Chamber Sociability Test | Ethovision XT (Noldus) |
Anxiety/Social Recognition
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Animal Behavior & Physiology Core | Karp RM 04014 | 3-Chamber Sociability Test |
Light Dark Box | Ethovision XT (Noldus) |
Anxiety
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Animal Behavior & Physiology Core | Karp RM 04014 | Light Dark Box |
Conditioned Fear | Ugo Basile |
Anxiety/Cognition
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Animal Behavior & Physiology Core | Karp RM 04006 | Conditioned Fear |
ActiTrack locomotor activity | Harvard Apparatus |
Voluntary activity
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Animal Behavior & Physiology Core | Karp RM 04016 | ActiTrack locomotor activity |
Grip Strength | tse-systems |
Motor Strength
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Animal Behavior & Physiology Core | Karp RM 04009 | Grip Strength |
DigiGait | Mouse Specifics |
Gait Analysis
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Animal Behavior & Physiology Core | Karp RM 04004 | DigiGait |
Treadmill | IITC Life Science |
Forced Activity
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Animal Behavior & Physiology Core | Karp RM 04018 | Treadmill |
Rotarod | Columbus Instrument |
Ataxia
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Animal Behavior & Physiology Core | Karp RM 04018 | Rotarod |
Pre-pulse Inhibition | Kinder Scientific |
Sensorimotor Gating
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Animal Behavior & Physiology Core | Karp RM 04006 | Acoustic Startle Reflex |
Acoustic Startle Reflex | Kinder Scientific |
Hearing reflex.
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Animal Behavior & Physiology Core | Karp RM 04006 | Acoustic Startle Reflex |
qOMR (Automated Optomotor) | Phenosys |
Visual acuity (brain stem reflex)
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Animal Behavior & Physiology Core | Karp RM 04004 | qOMR (Automated Optomotor) |
Optomotor | Celebral Mechanics |
Visual acuity (brain stem reflex)
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Animal Behavior & Physiology Core | Karp 04004 | Optomotor |
Activity Wheels | tse-systems |
Voluntary Activity
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Animal Behavior & Physiology Core | Karp 04016 | Activity Wheels |
Inframot (Infared Motion Sensor) | tse-systems |
Voluntary activity
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Animal Behavior & Physiology Core | Karp RM 04016 | Inframot (Infared Motion Sensor) |
4-Laser Fortessa (Analyzer) | BD LSRFortessa |
This Fortessa is an air-cooled four-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 12 colors + 2 Scatters. Hydrodynamically focused sample is interrogated within a quartz cuvette flow cell. Sample Aspiration rates at 12ul, 35ul, 60ul per minute.
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Flow Cytometry | Karp RM 8016 | 4-Laser Fortessa (Analyzer) |
5-Laser LSR II (Analyzer) | BD LSR II Sorp |
This LSR II is an air-cooled five-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 15 colors + 2 Scatters. The LSR II is equipped with high throughput sampler that provides rapid, fully automated sample acquisition. They are compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates.
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Flow Cytometry | Karp RM 8016 | 5 Laser LSR II (Analyzer) |
5-Laser Fortessa (Analyzer) | BD LSRFortessa Sorp |
This LSRFortessa is an air-cooled five-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 15 colors + 2 Scatters. They are compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates.
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Flow Cytometry | Karp RM 8016 | 5-Laser Fortessa (Analyzer) |
4-Laser FACSFusion | BD FACSARIA III |
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Most precise and sensitive digital PCR solution for a wide variety of applications.
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Flow Cytometry | Karp RM 6113 | 4-Laser FACSFusion (BL-2 Sorter) |
4-Laser FACSAria (BL2-Sorter) | BD FACSARIA II |
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Most precise and sensitive digital PCR solution for a wide variety of applications.
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Flow Cytometry | CLS 15 15037 | 4-Laser FACSAria (BL2-Sorter) |
6-Laser FACSAria (sorter) | BD FACSARIA II |
This instrument is capable of measuring 15 parameters, providing higher flexibility in experiment design for sorting. It is equipped with a 561 and 449 Laser line permitting detecting of Mcherry and cyan protein. This instrument provide all the great features we come to expect with the FACSAria sorters such as customized speed, multi population, variety of collection vessels and index sorting.
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Flow Cytometry | Karp RM 8113 | 6-Laser FACSAria (Sorter) |
3-Laser FACSAria (BL2-Sorter) | BD FACSARIA II |
The FACSAria cell sorter provides customized speed sorting. This instrument is bl2 certified and equipped with an Aerosol Management option for operator protection. Well designed for your research needs, the FACSAria allows simultaneous sorting 4 populations. Collection can be done in tubes or Plate. Index sorting now provides the option of match single cell profile with Fluorescence Intensity.
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Flow Cytometry | Karp RM 8113 | 3-Laser FACSAria (BL2-Sorter) |
GeneChip Microarray Scanner | Thermo Fisher/3000 7G |
Scan next-generation higher-density arrays, including SNP arrays with up to 900,000 SNPs, tiling arrays for transcription and all-exon arrays for whole-genome analysis.
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Molecular Genetics Core | CLS 16 16030.24 | GeneChip Microarray Scanner |
Agilent Microarray Scanner | Agilent/SureScan |
CGH & CGH+SNP Microarray Scanner. The Agilent SureScan Scanner provides a precise and efficient microarray scanner system for many research applications. Designed for research using microarrays, the scanner system provides a laser-induced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides. The Agilent SureScan Scanner provides a precise and efficient microarray scanner system for many research applications. Designed for research using microarrays, the scanner system provides a laser-induced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides.The microarray instrumentation measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays. The system also enhances productivity with continuous loading from a 24-slide cassette and automatic loading of data files into Feature Extraction Software. It also features built-in ozone protection to minimize signal degradation.
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Molecular Genetics | CLS 16 16030.23 | Agilent Microarray Scanner |
Combi | Thermo Fisher/Combi |
Housed in a sterile BL2 cabinet the combi takes advantage of a peristaltic pump to dispense cells into 96 and 384 cell culture plates. Dead volume if around 3-4ml with a dispense taking typically 10-12 seconds. Using this system minimizes varability in dispense volume and reduction in time to dispense multiple plates by hand.
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Human Neuron Core | CLS 12 121 | Combi |
Mantis | Formulatrix |
The Mantis is a medium throughput liquid handler with extremely low dead volume. The liquid handler uses either conventional pipette tips or reagents dispensed from 50 or 15ml Falcon tubes (for continous flow/bulk dispense). The Mantis is ideal for single well dispense of precious reagents. Fully programmable with a simple, yet powerful GUI. Examples of automated protocols using the Mantis within the core. NGS Library Prep (RNA-Seq), Magnetic Bead Dispensing, PCR Master Mix, Precious Reagent Dispensing, Cell Dispensing, PCR Setup.
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Human Neuron Core | CLS 12 160 |
Mantis |
Bravo, Benchcel and Plateloc | Agilent |
The Agilent Bravo is a versatile liquid handling platform capable of performing multiple tasks controlled from a simple graphical user interface. ADSF lab staff will assist investigators in the programming of custom protocols to facilitate reproducible pipetting for all experiments and assays. The 96-well microtip bravo head provides accurate liquid handling from 0.3-70µl. The Benchcel 4r microplate handler is a combination of automated microplate handling, and storage, that delivers speed and precision from the 4 intergrated microplate stacks and Plateloc microplate sealer. Intergration of the Bravo, Benchcel and Plateloc gives the ADSF a flexible system that can accommodate a wide variety of liquid handling tasks.
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Human Neuron Core | CLS 12 160 |
Bravo, Benchcel and Plateloc |
Spinnaker Robotic Arm | Thermo Fisher Canada/Spinnaker |
The Spinnaker robot arm works in conjunction with the two HCS imaging systems and the cytomat 5C hotelling incubator, to provide scheduled autonomous plate imaging capabilities. Book to reserve your inventory position in the cytomat 5C.
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Human Neuron Core | CLS 12 121 |
Spinnaker Robotic Arm |
Molecular Devices IXM-C High content screening with live cell imaging | Molecular Devices/IXM-C |
The ImageXpress® Micro Confocal High-Content Imaging System is a high content imaging system with confocality giving you the ultimate combination of speed, sensitivity and flexibility. High-resolution dual disk configuration with 60 micron confocal pinhole, 42 micron pinhole confocal and widefield modes. The Environmental Control Option for the ImageXpress Micro Confocal High-Content Imaging System can be configured to mimic conditions within a laboratory incubator (temperature control, gas mixture and humidity) to keep cells alive for long periods of time, making high-content screening kinetic and time lapse experiments a reality. During assay development, the Fluidics option facilitates compound addition and cell washing to characterize potency or to determine incubation times for higher throughput fixed time point assays.
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Human Neuron Core | CLS 12 121 |
Molecular Devices IXM-C High content screening with live cell imaging |
VIAFLO 96/384 liquid handler from Integra | VIAFLO 96/384 liquid handler from Integra |
Easy-to-use liquid handler that allows for exchange of liquid in 96-well or 384-well formats for changing media, adding compounds, setting up qPCR plates, making plate dilutions, washing plates, fixing plates, staining plates, etc.
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Human Neuron Core | CLS 12 163 |
VIAFLO 96/384 liquid handler from Integra |
IncuCyte S3 Live Imaging System from Sartorius | IncuCyte S3 |
The IncuCyte S3 is an industry leading screening platform for population based high-content analysis (HCA) that adds a new dimension of being able to image the same culture over time. The instrument fits conveniently into a standard CO2 incubator requiring no special adaptation, whilst accommodating up to 6 pieces of labware simultaneously (any plate type that conforms to the ANSI/Society for Lab Automation and Screening (SLAS) standard). The system possesses a fully automated, hands-free operation for periods exceeding 25 days, with automated imaging quality not deviating over these extended periods of time. This enables the end user to establish analysis of long-term cell cultures in vitro, a feature that is lacking in many competing platforms.
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Human Neuron Core | CLS 12 163 |
IncuCyte S3 Live Imaging System from Sartorius |
Hamamatsu Functional Drug Screening System FDSS 7000 EX | Hamamatsu FDSS7000EX |
The FDSS700ex is an advanced high throughput screening-dispense and imaging sytem for cell-based assays. The instrument optically detects intracellular reaction and biological signal transmissions to discover new lead compounds, which could be candidates for new drugs. Using adherent, suspension or primary cells the FDSS700 is capable of detecting intracellular Ca2+ ion, membrane potential, ion channel.
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Human Neuron Core | CLS 12 121 |
Hamamatsu Functional Drug Screening System FDSS 7000 EX |
ArrayScan™ XTI: High content screening with live cell imaging | ThermoFisher ArrayScan™ XTI |
The ArrayScan XTI (ThermoFisher Scientific) features a temperature- and CO2-controlled live cell chamber to assay cell biology over several days. An integrated liquid handler administers compounds well by well, capturing real-time changes in fluorescence with speeds up to 10 Hz with an enhanced. CCD camera and seven-color LED light source, using 6-well to 384-well labware. The instrument is ideally suited for automated image analysis of many cell types, particularly heterogeneous cell preparations that may require detec- tion of co-expressed markers to identify subsets of cells.
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Human Neuron Core | CLS 12 121 |
ArrayScan™ XTI: High content screening with live cell imaging |
Multi-electrode array (MEA) | Axion Biosystems Maestro Pro |
The Maestro multielectrode array technology (Axion Biosystems) enables analysis of neural networks and electroactive tissue at unprecedented throughput and scale. This system is ideal for large-scale cellular analysis in drug screening applications and for phenotyping human neurons.
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Human Neuron | CLS 12 12160.2 | Multi-electrode array (MEA) |
MaiTai Ti-Sapphire lasers (for Olympus MPE-RS & Zeiss 710) | Newport |
(available for use with the Olympus MPE-RS or the Zeiss 710)
|
Cellular Imaging Core | Varies |
MaiTai Ti-Sapphire lasers (for Olympus MPE-RS & Zeiss 710) |
IMARIS 9 visualization and analysis software | BitPlane |
3D/4D visualization and analysis for cell based segmentation, automated tracking, detection, interactive lineage trees and statistical tests (for customization in Matlab, Java or Python).
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Cellular Imaging Core | CLS 13 13035 |
IMARIS 9 visualization and analysis software |
CLARITYTissue clearing system | Life Canvas |
An ‘all-in-one’ instrument for tissue clearing. This system is able to process, and clarify a whole adult mouse brain in under 48 hours, allowing the sample to then be immunolabelled (IF/ICC, endogenous expression) and imaged downstream.
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Cellular Imaging Core | CLS 12 12145 |
Tissue clearing system |
TCS SP8-STED microscope (super-resolution) | Leica |
The base of this system is a standard confocal microscope suitable. Also available at the system is the Leica TCS SP8 STED ONE with white-light laser, for super-resolution STED imaging.
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Cellular Imaging Core | CLS 13 13035 | SP8-STED Super resolution microscope |
MPE-RS Confocal/Multiphoton - Laser Scanning | Olympus |
System capable of in vivo imaging in anesthesized and awake behaving mice and rats. Custom rig set-up available.
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Cellular Imaging Core | Enders SB-0068 |
MPE-RS Confocal/Multiphoton - Laser Scanning |
Ti Eclipse inverted microscope | Nikon |
Equipped with an incubator unit and automated stage, this system allows long term imaging of living samples.
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Cellular Imaging Core | CLS 13 13035 | Ti Eclipse inverted microscope |
E800 Transmitted Light/Fluorescence Microscope with oil objectives | Nikon |
Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence.
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Cellular Imaging Core | CLS 13 13035 | E800 Transmitted Light/Fluorescence Microscope with oil objectives |
80i Fluorescence Microscope with dry objectives | Nikon |
Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence.
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Cellular Imaging Core | CLS 12 12145 | 80i Fluorescence Microscope with dry objectives |
SteREO Discovery Dissecting Microscope | Zeiss |
Ideal for dissections this microscope uses a variable LED transmitted light base for even illumination of specimens, supplemental fibre optic illuminators, and a rotatable nosepiece with a 0.3 x, 0.5 x and 1 x objectives with programmable motorized zoom. Video capable for protocol development.
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Cellular Imaging Core | CLS 12 12145 | SteREO Discovery Dissecting Microscope |
LSM 710 Multiphoton Laser Scanning Confocal | Zeiss |
This instrument has a MaiTai tuneable IR laser and 2 channel GaAsP non-descanned detector for multiphoton imaging. Laser lines include 453, 488, 514, 561, 594 and 633.
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Cellular Imaging Core | CLS 13 13035 | LSM 710 Multiphoton Laser Scanning Confocal |
LSM 700 Laser Scanning Confocal | Zeiss |
This upright confocal is particularly suitable for combinations of GFP/YFP with the new generation of fluorescent proteins (e.g. mOrange/mCherry), and is fitted with a manual stage, facilitating easy location of samples/regions of interest.
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Cellular Imaging Core | CLS 13 13035 |
LSM 700 Laser Scanning Confocal |
3-Laser MACSVYB (Analyzer) | Miltenyi MACSVYB |
The MACSQuant VYB is a versatile 3 laser, 10-parameter flow cytometer that has enhanced capabilities for detection of fluorescent protein reporters. It is a fully operated instrument capable of running multi-tube, 96 and 384 plates. |
Flow Cytometry | Karp RM 8018 | 3-Laser MACSVYB |
ADVIA 2120 Hematology Analyzer | Sysmex Advia 2120 |
The ADVIA is a hematology analyzer for measure blood count. It provides the flexibility of measuring multiple diff species and complete blood count with differential and reticulocyte count. |
Flow Cytometry | Karp RM 8018 |
ADVIA 2120 Hematology Analyzer |
6-Laser FACSAria (Sorter) | BD FACSARIA II |
This instrument is capable of measuring 15 parameters, providing higher flexibility in experiment design for sorting. It is equipped with a 561 and 449 Laser line permitting detecting of Mcherry and cyan protein. This instrument provide all the great features we come to expect with the FACSAria sorters such as customized speed, multi population, variety of collection vessels and index sorting.
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Molecular Genetics Core | Karp 8 RM 8113 | 6-Laser FACSAria |
MACS Quant VYB | MACS QUANT ANALYZER |
MACSVYB is an air-cooled three-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 8 colors + 2 Scatters It is equipped with high throughput sampler that provides rapid, fully automated sample acquisition. They are compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates
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Flow Cytometry | Karp RM 08016 |
MACS Quant VYB |
4 Laser ARIA FUSION | BD FACS FUSION |
This Aria is located in CLS building room 15037. It is inside a self-contain hood allowing for sorting of Primary Human in a complete sterile environment. The CLS-Aria is a bench-top high-speed sorter with fixed- alignment cuvette flow cell for high fluorescence sensitivity.
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Flow Cytometry | Karp RM 6113 | 4 Laser ARIA FUSION |
4 Laser CLS-ARIA | BD FACS ARIA II |
This Aria is located in CLS building room 15037. It is inside a self-contain hood allowing for sorting of Primary Human in a complete sterile environment. The CLS-Aria is a bench-top high-speed sorter with fixed- alignment cuvette flow cell for high fluorescence sensitivity. |
Flow Cytometry | CLS 15 15037 | 4 Laser CLS-ARIA |
6 Laser Self-Aria | BD FACS ARIA II |
This Aria can be operated by our staff or users who have been trained and certified by our facility. It is bench-top high-speed sorter with fixed- alignment cuvette flow cell for high fluorescence sensitivity. |
Karp Rm 8113 |
6 Laser Self-Aria |
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Agilent Bioanalyzer | Agilent/Bioanalyzer 2100 |
The 2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of biomolecules. The 2100 Bioanalyzer instrument, together with the 2100 Expert Software and Bioanalyzer assays, provide highly precise analytical evaluation of various samples types in many workflows, including next generation sequencing (NGS), gene expression, biopharmaceutical, and gene editing research. Digital data is provided in a timely manner and delivers objective assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for an accurate result, and the data may be exported in a many different formats for ease-of-use.
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Molecular Genetics Core | CLS 16 16030.23 | Agilent Bioanalyzer |
Perkin Elmer Multilabel Counter | Perkin Elmer/Victor3 |
Fluorescence detection for quantification using Picogreen or Ribogreen.
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Molecular Genetics Core | CLS 16 16030.22 | Perkin Elmer Multilabel Counter |
Fluidigm Biomark high-performance PCR system | Fluidigm/Biomark |
Microfluidics technology to process samples at nanoliter-scale volumes for gene expression, genotyping, sample identification, copy number variation analysis and digital PCR. Ideal for labs performing PCR on hundreds to thousands of samples. Provides a wide range of integrated fluidic circuit (IFC) formats available, scaling from mid- to high throughput on a single system is easily achievable. Available controller are HX, MX, and RX.
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Molecular Genetics Core | CLS 16 16030.21 | Fluidigm Biomark high-performance PCR system |