Boston Children's Core Equipments

Equipment Name Make/Model Functionality Core Facility Location Equipment URL
Tokai Hit Environmental Chamber for Keyence BZ-X700 STR
CO2 and Temp control for live cell monitoring on Keyence epifluorescent scope
Molecular Genetics Core
Agilent 4200 Tapestation 4200
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.
Molecular Genetics Core Enders 1317 Agilent 4200 TapeStation
nCounter SPRINT Profiler Nanostring/ SPRINT Profiler
Fully automated analysis platform for Nanostring’s nCounter technology, which allows for molecular quantification of a diverse range of DNA, RNA and protein targets through the use of fluorescent probes. Capable of multiplex analysis of up to 800 targets.
Molecular Genetics Core CLS 16049
Chromium Controller 10x Genomics/Chromium Controller
Partitioning device used to isolate single cells from a cell suspension in “Gel Bead-in-Emulsion” droplets, where nucleic acid of interest is extracted and tagged with a cell-specific barcode for downstream Next-Gen Sequencing library prep and sequencing.
Molecular Genetics Core CLS 16030.23

<a href="https://www.10xgenomics.com/products/single-cell-gene-expression"> Chromium Controller
</a>

4200 TapeStation Agilent/4200 TapeStation
Fully automated electrophoresis device for RNA and DNA sample quality assessment. Capable of assessing intactness of extracted genomic DNA or RNA, as well as size and concentration of either nucleic acid. Especially useful for Next-Gen Sequencing quality control, such as library-prep QC.
Molecular Genetics Core CLS 16030.23 4200 TapeStation
NanoDrop 8000 Spectrophotometer (ECRAC Cycle VII) ThermoFisher/NanoDrop 8000
Microvolume UV-Vis and Fluorescence spectrophotometer used for nucleic acid and protein quantification and quality assessment. Additionally capable of Microarray fluorescent labeling QC analysis, as well as Pierce 660, Bradford, BCA, Lowry assays. 8000 model has 8 channels improving speed.
Molecular Genetics Core CLS 16030.23
SeqStudio Genetic Analyzer Applied Biosystems/SeqStudio
Capillary electrophoresis system capable of Sanger Sequencing analysis (for NGS confirmation, indel detection, SNP detection, genome editing verification, microbial identification, etc…) and fragment analysis (ie microsatellite analysis).

Molecular Genetics: <a href="https://www.childrenshospital.org/research/cores/molecular-genetics-core"> Molecular Genetics Core </a>

CLS 16030.21
Centrifuge Eppendorf 5430R
Works with 50 mL conical tubes and 1.5 ml microcentrifuge tubes
CLS 13060.8, CLS 13060.9, CLS 13060.10 Centrifuge
DiSPIM Lightsheet microscope ASI
Inverted dual objective Lightsheet microscope system allows rapid 3-dimensional live and fixed imaging of cells, tissues and organoids. The system is equipped with piezo-controlled dual objectives (20x air, or 40x water), four solid state lasers with AOTF control, X-Y-Z piezo driven motorized stage, specialized chambers, computer and high-resolution monitor. This system is additionally configured for live inverted ratio epifluorescence imaging with a DG4 high-speed switching xenon light source and digital CMOS camera.
Cell Function and Imaging Core Enders 613 DiSPIM Lightsheet microscope
Seahorse Xfe 96 Analyzer Agilent
Analyzer measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. These rates are key indicators of mitochondrial respiration and glycolysis. They provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
Cell Function and Imaging Core Enders 622 Seahorse Xfe 96 Analyzer
FACSMelody Cell Sorter BD Bioscience
This cell-sorting technology allows researchers to simultaneously isolate two cell populations into separate tubes or deposit single or multiple target cells into 96- or 384-well plates.
Cell Function and Imaging Core Enders 613

FACSMelody Cell Sorter

FACSCanrto II BD Bioscience
Analysis of cell surface or intracellular proteins or other “markers,” intracellular cytokine production and receptor-mediated endocytosis, sorting, and recycling.
Cell Function and Imaging Core Enders 613 FACSCanrto II
BD LSRFortess BD Bioscience
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.
Cell Function and Imaging Core Enders 613 BD LSRFortess
Kinetic Imaging Cytometer (KIC) Vala
High content live cell imager capable of electrical or optical stimulation and high-speed acquisition with an analysis package designed for quantifying parameters from individual cells within a well
Cell Function and Imaging Core Enders 1250

Kinetic Imaging Cytometer (KIC)

FV300R Olympus
Resonant scanning confocal microscope equipped with 4 laser lines (405, 488, 514, and 633 nm), hybrid galvo and fast resonant scanning capabilities, ultra-sensitive GaAsP detectors with full spectral imaging, motorized XYZ stage, air and silicone oil objectives, up to 100X, and advanced image analysis with the Olympus CellSens software
Cell Function and Imaging Core Enders 1347

FV300R

BZ-X700 Keyence
Epifluorescent, brightfield, and phase imaging, image stitching for capturing images larger than one field, automated multiwell plate image sample, structured illumination, live cell imaging with video rate capture and environmental controls and built-in image analysis functions.
Cell Function and Imaging Core Enders 1347 BZ-X700
Cytation 5 Biotek
Automated imaging system with filter cubes for DAPI, Alexa 488, Alexa 568 and Alexa 647 as well as transmitted light. It can accept slides, multiwell plates and 35mm dishes, has incubation capabilities including CO2 and software for automated capture, image processing and analysis.
Cell Function and Imaging Core Enders 620 Cytation 5
BX41 upright Histology Microscope Olympus
Upright microscope with a Pixelink PL-D682CU color CMOS camera for brightfield and epifluorescence imaging.
Cell Function and Imaging Core Enders 603

BX41 upright Histology Microscope

Axiovert Spinning Disk Confocal Zeiss
Spinning Disk Confocal microscope with 3 laser lines (488, 568, 640), LED epifluorescence and halogen brightfield light sources, temperature controlled enclosure, motorized XY stage, piezo-controlled Z-stage, enabled for confocal, epifluorescence and brightfield fixed and live cell imaging
Cell Function and Imaging Core Enders 613

Axiovert Spinning Disk Confocal

LSM 880 Zeiss
Laser Scanning Confocal microscope with 6 laser lines (405/458/488/514/561/633nm), Fast Airyscan for near super-resolution confocal imaging, LED epifluorescence and halogen brightfield light sources, temperature controlled enclosure, motorized XY stage, piezo-controlled Z-stage, enabled for confocal, brightfield, and DIC, fixed and live cell imaging.
Cell Function and Imaging Core Enders 613 LSM 800
Multiphoton Microscope Olympus FV1200 MPE Multiphoton Microscope (with BX 61 microscope setup)
Multiphoton imaging of fixed or live samples; specialization in deep tissue and Intravital Imaging of live mice
PCMM Microscopy Core 200 Longwood Ave, WAB Rm247a

Multiphoton Microscope

Confocal Microscope Olympus FV1000 Confocal Microscope (with IX81 microscope setup)
Confocal imaging of fixed or live samples
PCMM Microscopy Core CLS 3rd Floor, Rm 03123 Confocal Microscope
Sony MA900 Sorter MA900
Cell Sorting
hESC Core Facility RB 5214 Sony MA900 Sorter
CV7000 Imager CV7000
High-throughout high-content confocal fluorescence imaging.
hESC Core Facility RB05214

CV7000 Imager

Motion tracking system KinetiCor
Prospective motion correction of MRI images
For more information contact Simon Warfield at Simon.Warfield@childrens.harvard.edu
Boston

Motion tracking system

fMRI equipment NordicNeuroLab fMRI equipment
functional MRI data collection
For more information contact Simon Warfield at Simon.Warfield@childrens.harvard.edu
Boston Waltham

fMRI equipment

ImageStream Amnis ImageStream MarkX
Amnis ImageStream MarkX
Flow Cytometry PCMM ARM 231, 200 Longwood Ave ImageStream
BL2 Aria Cell Sorter BD FACS Aria II
BL2 Cell Sorter
Flow Cytometry PCMM ARM 239, 200 Longwood Ave. BL2 Aria Cell Sorter
Stony Cell Sorter Sony SH800Z
Cell Sorter
Flow Cytometry PCMM WAB 109, 200 Longwood Ave.

Stony Cell Sorter

CLSB-Canto Flow Cytometry Analyzer BD FACS CantoII
Flow Cytometry Analyzer with Plate Reader
Flow Cytometry PCMM CLSB 3034, 3 Blackfan Circle CLSB-Canto Flow Cytometry Analyzer
AR-CantoII Flow Cytometry Analyzer BD FACS Cantoll
Flow Cytometry Analyzer
Flow Cytometry PCMM Arm 239, 200 Longwood Ave

AR-CantoII Flow Cytometry Analyzer

Dual inverted selective plane illumination microscope ASI/DiSPIM
Light Sheet Microscope, primary niche is rapid low light dose imaging of 3D objects.
Cellular Imaging Core Enders 613 Dual inverted selective plane illumination microscope
Thermo Fisher Stericult double stacked relative humidity-controlled incubator Human Neuron Core Thermo Fisher Stericult double stacked relative humidity-controlled incubator
Neurolucida and Stereo Investigator Nikon
Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence.
Cellular Imaging Core CLS 12 12145 Nikon 80i with Neurolucida and Stereo Investigator
Biorad Droplet Digital PCR System BIORAD/QX200 with Auto DG
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.
Molecular Genetics CLS 16 16030.22 Biorad Droplet Digital PCR System
Magnetic Resonance
For more information contact Simon Warfield at Simon.Warfield@childrens.harvard.edu
Magnetic resonance
VisualSonics Vevo 2100 ultrasound
The laboratory is equipped with a VisualSonics Vevo 2100 small animal ultrasound system. This is a high-resolution instrument (30 microns) that can also image at high speed (up to 100 frames per second). It is capable of imaging in 3D mode as well as M mode, and can also provide pulsed wave Doppler imaging. The device is equipped with two transducers (25 and 40 MHz) and the Vevo Integrated Rail System, which facilitates alignment of the mouse and the probe as well as image-guided injection, when necessary. The table is heated and includes sensor pads for monitoring the animal during examination. The system is routinely used for cardiac imaging in small animals.
For more information contact Simon Warfield at Simon.Warfield@childrens.harvard.edu

VisualSonics Vevo 2100 ultrasound

Siemens microCAT II
The Siemens microCAT II is a high-resolution (27 microns in standard mode and 15 microns in high-resolution mode) CT scanner designed specifically for imaging small animals such as mice and rats. The radiation detector is configurable such that, for mice, the transaxial field of view is 5.4 cm and the axial field of view is 8 cm; whereas, for rats, the transaxial field of view is 8 cm and the axial field of view is 5.4 cm. We have utilized microCT for tumor imaging, for anatomical correlation for microPET studies, and for ex vivo imaging (e.g., structural analysis of porcine cardiac valves)
For more information contact Simon Warfield at Simon.Warfield@childrens.harvard.edu

Siemens microCAT II

Siemens Focus 120 microPET
"The Siemens Focus 120 MicroPET is a high-resolution, small animal PET scanner that combines high spatial resolution (<1.3 mm) and high sensitivity (>7%) with a bore size (12 cm diameter and 7.6 axial length) that is optimized for imaging mice and rats. Among commercially available radioactive agents, we are routinely imaging with 18F 2-fluoro-2-deoxy-D-glucose (FDG) and 18F sodium fluoride. We have also carried out some microPET studies with 18F fluorothymidine (FLT) provided by the Division of Nuclear Medicine at Massachusetts General Hospital; we have validated the synthesis of 18F FHBG and have carried out preliminary studies with this tracer."
For more information contact Simon Warfield at Simon.Warfield@childrens.harvard.edu
Siemens Focus 120 microPET
Von Frey Stoelting
Mechanical allodynia threshold
Animal Behavior & Physiology Core Karp RM 04009 Von Frey
Thermal Gradient Bioseb
Threshold heat preference
Animal Behavior & Physiology Core Karp RM 04016 Thermal Gradient
NextSeq 500 Illumina/NextSeq 500
Provides high quality, high coverage genome with streamlined library preparation using high quality TruSeq library preparation kits.  Single, paired-end, and indexed sequencing with accuracy of > 75% of sequenced bases over Q30.  Enables researchers to interrogate simple prokaryotic and complex eukaryotic genomes quickly and efficiently.
Molecular Genetics CLS 16 16054 NextSeq 500
CFX384 Touch Real-Time PCR Detection System Biorad/CFX384
The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.
Molecular Genetics CLS 16 16054 CFX384 Touch Real-Time PCR Detection System
ABI 3730 DNA Analyzer Thermo Fisher/SeqStudio
The 48-capillary 3730 DNA Analyzer is the Gold Standard in medium-to-high throughput genetic analysis. Use the 3730 DNA Analyzer for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample.
Molecular Genetics CLS 12 ABI 3730 DNA Analyzer
CFX 96 and CFX384 Touch Real-Time PCR Detection System Biorad/CFX96
The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.
Molecular Genetics CLS 16 16054 4CFX 96 and CFX384 Touch Real-Time PCR Detection System
Bionano Saphyr Bionano/Saphyr
The Saphyr System by Bionano Genomics is a whole genome imaging tool for high-speed, high-throughput structural variant detection and analysis with exceptional sensitivity and specificity. Wide range of applications includes: undiagnosed genetic disorders, hematologic malignancies, gene discovery and therapy, cell line stability, solid tumor research, genetic engineering studies, evolutionary biology, and reference genome assembly.  Resolve large-scale structural variations missed by next-generation sequencing systems. Detect structural variants with high specificity and sensitivity.
Molecular Genetics CLS 12 Bionano Saphyr
nCounter® SPRINT Profiler   Nanostring/Sprint Profiler
The nCounter® SPRINT Profiler makes access to NanoString’s molecular barcoding technology more affordable than ever before for RNA, DNA, and protein profiling applications. Digitally examine multiple pathways in a single tube in an extraction-free workflow. Perform your own data analysis using the  nSolver™ Analysis Software. Compatible with difficult sample types, including FFPE tissue. Highly multiplexed analysis of basic cancer biology and pathway deregulation activity. 
Molecular Genetics CLS 12 nCounter® SPRINT Profiler
Stereotaxic frames Harvard Apparatus Animal Behavior & Physiology Core Karp RM 04002/17 Stereotaxic Frames
Dissecting microscopes Leica/Zeiss
Lecia/Zeiss
Animal Behavior & Physiology Core Karp RM 04002/17 Leica/Zeiss
In vivo microdialysis BASi Raturn and BAS HoneyComb
Microdialysis
Animal Behavior & Physiology Core In vivo microdialysis
Open flow microperfusion BASi Microperfusion
Microdialysis
Animal Behavior & Physiology Core Karp 04009 Open flow microperfusion
In vivo calcium imaging Neurophotometrics
Multi-fiber photometry
Animal Behavior & Physiology Core Karp 4 Portable In vivo calcium imaging
Calcium imaging and Optogenetics Calcium Imaging and Optogenetics
Microendoscope fitted with fiber-coupled laser and LED light sources that is used in conjunction with implantable gradient-index (GRIN) lenses
Animal Behavior & Physiology Core Karp 4 Portable Calcium imaging and Optogenetics
Whole Body Plethysmography SCIREQ (EMKA Technologies)
Breathing measures, apneas
Animal Behavior & Physiology Core Karp 04009 Whole Body Plethysmography
ECGenie Mouse Specifics
Conscious, unrestrained ECG
Animal Behavior & Physiology Core Karp 04009 ECGenie
TMS with electromyography and/or accelerometry TMS with electromyography and/or accelerometry
In vivo cortical stimulation for translational experiments aimed to measure cortical excitability, cortical inhibition, cortical plasticity, motor evoked potentials
Animal Behavior & Physiology Core CLS 14 TMS with electromyography and/or accelerometry
Subdermal wire electrode EEG Cadwell Animal Behavior & Physiology Core Karp RM 04013 Subdermal wire electrode EEG
Tethered EEG Pinnacle Technology
Sleep architecture analysis for up to 5 days
Animal Behavior & Physiology Core Karp RM 04013 Tethered EEG, multichannel
Wireless EEG NEW FORMULA TESTING
Seizure susceptibility, epileptiform abnormalities, circadian disturbances, actigraphy, auditory evoked response potentials; continuous monitoring available for up to 4 months
Animal Behavior & Physiology Core Karp RM 04013 Wireless EEG
Hot plate Bioseb
Noxious heat sensitivity
Animal Behavior & Physiology Core Karp RM 04016 Hot plate
Operant Touchscreens Lafayette Instruments
Attention/working memory/flexibility
Animal Behavior & Physiology Core Karp RM 04015 Operant Touchscreens
Morris Water Maze Ethovision XT (Noldus)
Spatial short term working memory
Animal Behavior & Physiology Core Karp RM 04008 Morris Water Maze
Water T Maze Cognitive Flexibility
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.
Animal Behavior & Physiology Core Karp RM 04014 Water T Maze
Y Maze Ethovision XT (Noldus)
Spatial short term working memory
Animal Behavior & Physiology Core Karp RM 04014 Y Maze
Novel Object Recognition Ethovision XT (Noldus)
Short term working memory
Animal Behavior & Physiology Core Karp RM 04014 Novel Object Recognition
Contextual Fear Conditioning Stoelting (ANY-maze)
Anxiety/Cognition
Animal Behavior & Physiology Core Karp RM 04014 Contextual Fear Conditioning"
Ultrasonic Vocalizations Sonotrack, Metris
Communications
Animal Behavior & Physiology Core Karp RM 04006 BIORAD Droplet Digital PCR System
Open field/holeboards Kinder Scientific
Anxiety Activity
Animal Behavior & Physiology Core Karp RM 04016 Open field/holeboards
Tail Suspension Ethovision XT (Noldus)
Response to Stress
Animal Behavior & Physiology Core Karp RM 04016 Tail Suspension
Forced Swim Ethovision XT (Noldus)
Response to Stress
Animal Behavior & Physiology Core Karp RM 04016 Forced Swim
Conditioned Place Preference Ethovision XT (Noldus)
Emotional response to stimulus/condition
Animal Behavior & Physiology Core Karp RM 04014 Conditioned Place Preference
Elevated Pluz Maze
Anxiety
Animal Behavior & Physiology Core Elevated Plus Maze
3- Chamber Sociability Test Ethovision XT (Noldus)
Anxiety/Social Recognition
Animal Behavior & Physiology Core Karp RM 04014 3-Chamber Sociability Test
Light Dark Box Ethovision XT (Noldus)
Anxiety
Animal Behavior & Physiology Core Karp RM 04014 Light Dark Box
Conditioned Fear Ugo Basile
Anxiety/Cognition
Animal Behavior & Physiology Core Karp RM 04006 Conditioned Fear
ActiTrack locomotor activity Harvard Apparatus
Voluntary activity
Animal Behavior & Physiology Core Karp RM 04016 ActiTrack locomotor activity
Grip Strength tse-systems
Motor Strength
Animal Behavior & Physiology Core Karp RM 04004 Grip Strength
DigiGait Mouse Specifics
Gait Analysis
Animal Behavior & Physiology Core Karp RM 04004 DigiGait
Treadmill IITC Life Science
Forced Activity
Animal Behavior & Physiology Core Karp RM 04018 Treadmill
Rotarod Columbus Instrument
Ataxia
Animal Behavior & Physiology Core Karp RM 04018 Rotarod
Pre-pulse Inhibition Kinder Scientific
Sensorimotor Gating
Animal Behavior & Physiology Core Karp RM 04006 Acoustic Startle Reflex
Acoustic Startle Reflex Kinder Scientific
The QX200 Droplet Digital PCR (ddPCR) Hearing Reflex
Animal Behavior & Physiology Core Karp RM 04006 Acoustic Startle Reflex
qOMR (Automated Optomotor) Phenosys
Visual acuity (brain stem reflex)
Animal Behavior & Physiology Core Karp RM 04004 qOMR (Automated Optomotor)
Optomotor Celebral Mechanics
Visual acuity (brain stem reflex)
Animal Behavior & Physiology Core Karp 04004 Optomotor
Activity Wheels tse-systems
Voluntary Activity
Animal Behavior & Physiology Core Karp 04016 Activity Wheels
Inframot (Infared Motion Sensor) tse-systems
Voluntary activity
Animal Behavior & Physiology Core Karp RM 04016 Inframot (Infared Motion Sensor)
4-Laser Fortessa (Analyzer) BD LSRFortessa
This Fortessa is an air-cooled four-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 12 colors + 2 Scatters. Hydrodynamically focused sample is interrogated within a quartz cuvette flow cell. Sample Aspiration rates at 12ul, 35ul, 60ul per minute.
Flow Cytometry Karp RM 8016 4-Laser Fortessa (Analyzer)
5-Laser LSR II (Analyzer) BD LSR II Sorp
This LSR II is an air-cooled five-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 15 colors + 2 Scatters. The LSR II is equipped with high throughput sampler that provides rapid, fully automated sample acquisition. They are compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates.
Flow Cytometry Karp RM 8016 5 Laser LSR II (Analyzer)
5-Laser Fortessa (Analyzer) BD LSRFortessa Sorp
This LSRFortessa is an air-cooled five-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 15 colors + 2 Scatters. They are compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates.
Flow Cytometry Karp RM 8016 5-Laser Fortessa (Analyzer)
4-Laser FACSFusion BD FACSARIA III
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.
Flow Cytometry Karp RM 6113 4-Laser FACSFusion (BL-2 Sorter)
4-Laser FACSAria (BL2-Sorter) BD FACSARIA II
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. Most precise and sensitive digital PCR solution for a wide variety of applications.
Flow Cytometry CLS 15 15037 4-Laser FACSAria (BL2-Sorter)
6-Laser FACSAria (sorter) BD FACSARIA II
This instrument is capable of measuring 15 parameters, providing higher flexibility in experiment design for sorting. It is equipped with a 561 and 449 Laser line permitting detecting of Mcherry and cyan protein. This instrument provide all the great features we come to expect with the FACSAria sorters such as customized speed, multi population, variety of collection vessels and index sorting.
Flow Cytometry Karp RM 8113 6-Laser FACSAria (Sorter)
3-Laser FACSAria (BL2-Sorter) BD FACSARIA II
The FACSAria cell sorter provides customized speed sorting. This instrument is bl2 certified and equipped with an Aerosol Management option for operator protection.  Well designed for your research needs, the FACSAria allows simultaneous sorting 4 populations.  Collection can be done in tubes or Plate.  Index sorting now provides the option of match single cell profile with Fluorescence Intensity. 
Flow Cytometry Karp RM 8113 3-Laser FACSAria (BL2-Sorter)
GeneChip Microarray Scanner Thermo Fisher/3000 7G
Scan next-generation higher-density arrays, including SNP arrays with up to 900,000 SNPs, tiling arrays for transcription and all-exon arrays for whole-genome analysis.
Molecular Genetics Core CLS 16 16030.24 GeneChip Microarray Scanner
Agilent Microarray Scanner Agilent/SureScan
CGH & CGH+SNP Microarray Scanner. The Agilent SureScan Scanner provides a precise and efficient microarray scanner system for many research applications. Designed for research using microarrays, the scanner system provides a laser-induced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides. The Agilent SureScan Scanner provides a precise and efficient microarray scanner system for many research applications. Designed for research using microarrays, the scanner system provides a laser-induced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides.The microarray instrumentation measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays. The system also enhances productivity with continuous loading from a 24-slide cassette and automatic loading of data files into Feature Extraction Software. It also features built-in ozone protection to minimize signal degradation. 
Molecular Genetics CLS 16 16030.23 Agilent Microarray Scanner
QIAGEN Automated Nucleic Acid Extraction Qiagen/QIAcube
For fully automated nucleic acid extraction with QIAGEN spin-column kits. Automates over 80 QIAGEN kits with over 3000 protocols. Allows automated DNA, RNA and protein sample processing. Eliminates manual processing steps.Generates standardized results. 
Molecular Genetics Core CLS 16 16030.23  QIAGEN Automated Nucleic Acid Extraction
Combi Thermo Fisher/Combi
Housed in a sterile BL2 cabinet the combi takes advantage of a peristaltic pump to dispense cells into 96 and 384 cell culture plates. Dead volume if around 3-4ml with a dispense taking typically 10-12 seconds. Using this system minimizes varability in dispense volume and reduction in time to dispense multiple plates by hand.
Human Neuron Core CLS 12 121 Combi
Mantis Formulatrix
The Mantis is a medium throughput liquid handler with extremely low dead volume. The liquid handler uses either conventional pipette tips or reagents dispensed from 50 or 15ml Falcon tubes (for continous flow/bulk dispense). The Mantis is ideal for single well dispense of precious reagents. Fully programmable with a simple, yet powerful GUI. Examples of automated protocols using the Mantis within the core. NGS Library Prep (RNA-Seq), Magnetic Bead Dispensing, PCR Master Mix, Precious Reagent Dispensing, Cell Dispensing, PCR Setup.
Human Neuron Core CLS 12 160

Mantis

Bravo, Benchcel and Plateloc  Agilent
The Agilent Bravo is a versatile liquid handling platform capable of performing multiple tasks controlled from a simple graphical user interface. ADSF lab staff will assist investigators in the programming of custom protocols to facilitate reproducible pipetting for all experiments and assays. The 96-well microtip bravo head provides accurate liquid handling from 0.3-70µl. The Benchcel 4r microplate handler is a combination of automated microplate handling, and storage, that delivers speed and precision from the 4 intergrated microplate stacks and Plateloc microplate sealer. Intergration of the Bravo, Benchcel and Plateloc gives the ADSF a flexible system that can accommodate a wide variety of liquid handling tasks.
Human Neuron Core CLS 12 160

Bravo, Benchcel and Plateloc 

Spinnaker Robotic Arm Thermo Fisher Canada/Spinnaker
The Spinnaker robot arm works in conjunction with the two HCS imaging systems and the cytomat 5C hotelling incubator, to provide scheduled autonomous plate imaging capabilities. Book to reserve your inventory position in the cytomat 5C.
Human Neuron Core CLS 12 121

Spinnaker Robotic Arm

Molecular Devices IXM-C High content screening with live cell imaging Molecular Devices/IXM-C
The ImageXpress® Micro Confocal High-Content Imaging System is a high content imaging system with confocality giving you the ultimate combination of speed, sensitivity and flexibility. High-resolution dual disk configuration with 60 micron confocal pinhole, 42 micron pinhole confocal and widefield modes. The Environmental Control Option for the ImageXpress Micro Confocal High-Content Imaging System can be configured to mimic conditions within a laboratory incubator (temperature control, gas mixture and humidity) to keep cells alive for long periods of time, making high-content screening kinetic and time lapse experiments a reality. During assay development, the Fluidics option facilitates compound addition and cell washing to characterize potency or to determine incubation times for higher throughput fixed time point assays.
Human Neuron Core CLS 12 121

Molecular Devices IXM-C High content screening with live cell imaging

VIAFLO 96/384 liquid handler from Integra VIAFLO 96/384 liquid handler from Integra
Easy-to-use liquid handler that allows for exchange of liquid in 96-well or 384-well formats for changing media, adding compounds, setting up qPCR plates, making plate dilutions, washing plates, fixing plates, staining plates, etc.
Human Neuron Core CLS 12 163

VIAFLO 96/384 liquid handler from Integra

IncuCyte S3 Live Imaging System from Sartorius IncuCyte S3 
The IncuCyte S3 is an industry leading screening platform for population based high-content analysis (HCA) that adds a new dimension of being able to image the same culture over time. The instrument fits conveniently into a standard CO2 incubator requiring no special adaptation, whilst accommodating up to 6 pieces of labware simultaneously (any plate type that conforms to the ANSI/Society for Lab Automation and Screening (SLAS) standard). The system possesses a fully automated, hands-free operation for periods exceeding 25 days, with automated imaging quality not deviating over these extended periods of time. This enables the end user to establish analysis of long-term cell cultures in vitro, a feature that is lacking in many competing platforms. 
Human Neuron Core CLS 12 163

IncuCyte S3 Live Imaging System from Sartorius

Hamamatsu Functional Drug Screening System FDSS 7000 EX Hamamatsu FDSS7000EX 
The FDSS700ex is an advanced high throughput screening-dispense and imaging sytem for cell-based assays. The instrument optically detects intracellular reaction and biological signal transmissions to discover new lead compounds, which could be candidates for new drugs. Using adherent, suspension or primary cells the FDSS700 is capable of detecting intracellular Ca2+ ion, membrane potential, ion channel. 
Human Neuron Core CLS 12 121

Hamamatsu Functional Drug Screening System FDSS 7000 EX

ArrayScan™ XTI: High content screening with live cell imaging  ThermoFisher ArrayScan™ XTI 
The ArrayScan XTI (ThermoFisher Scientific) features a temperature- and CO2-controlled live cell chamber to assay cell biology over several days. An integrated liquid handler administers compounds well by well, capturing real-time changes in fluorescence with speeds up to 10 Hz with an enhanced. CCD camera and seven-color LED light source, using 6-well to 384-well labware. The instrument is ideally suited for automated image analysis of many cell types, particularly heterogeneous cell preparations that may require detec- tion of co-expressed markers to identify subsets of cells.
Human Neuron Core CLS 12 121

ArrayScan™ XTI: High content screening with live cell imaging 

Multi-electrode array (MEA) Axion Biosystems Maestro Pro
The Maestro multielectrode array technology (Axion Biosystems) enables analysis of neural networks and electroactive tissue at unprecedented throughput and scale. This system is ideal for large-scale cellular analysis in drug screening applications and for phenotyping human neurons.
Human Neuron CLS 12 12160.2 Multi-electrode array (MEA)
MaiTai Ti-Sapphire lasers (for Olympus MPE-RS & Zeiss 710)  Newport
(available for use with the Olympus MPE-RS or the Zeiss 710)
Cellular Imaging Core Varies

MaiTai Ti-Sapphire lasers (for Olympus MPE-RS & Zeiss 710)

IMARIS 9 visualization and analysis software BitPlane
3D/4D visualization and analysis for cell based segmentation, automated tracking, detection, interactive lineage trees and statistical tests (for customization in Matlab, Java or Python).
Cellular Imaging Core CLS 13 13035

IMARIS 9 visualization and analysis software

CLARITYTissue clearing system Life Canvas
An ‘all-in-one’ instrument for tissue clearing. This system is able to process, and clarify a whole adult mouse brain in under 48 hours, allowing the sample to then be immunolabelled (IF/ICC, endogenous expression) and imaged downstream.
Cellular Imaging Core CLS 12 12145

Tissue clearing system

TCS SP8-STED microscope (super-resolution) Leica 
The base of this system is a standard confocal microscope suitable. Also available at the system is the Leica TCS SP8 STED ONE with white-light laser, for super-resolution STED imaging. 
Cellular Imaging Core CLS 13 13035 SP8-STED Super resolution microscope 
MPE-RS Confocal/Multiphoton - Laser Scanning Olympus
System capable of in vivo imaging in anesthesized and awake behaving mice and rats. Custom rig set-up available.
Cellular Imaging Core Enders SB-0068

MPE-RS Confocal/Multiphoton - Laser Scanning

Array Tomography Imaging System Array Tomography Imaging System
Able to obtain quantitative, high-resolution, large-field volumetric imaging of molecular architecture and cellular ultrastructure.
Cellular Imaging Core CLS 13 13035

Array Tomography Imaging System

Ti Eclipse inverted microscope Nikon
Equipped with an incubator unit and automated stage, this system allows long term imaging of living samples.
Cellular Imaging Core CLS 13 13035 Ti Eclipse inverted microscope
E800 Transmitted Light/Fluorescence Microscope with oil objectives Nikon
Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence.
Cellular Imaging Core CLS 13 13035 E800 Transmitted Light/Fluorescence Microscope with oil objectives
80i Fluorescence Microscope with dry objectives Nikon
Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence.  
Cellular Imaging Core CLS 12 12145 80i Fluorescence Microscope with dry objectives
SteREO Discovery Dissecting Microscope Zeiss
Ideal for dissections this microscope uses a variable LED transmitted light base for even illumination of specimens, supplemental fibre optic illuminators, and a rotatable nosepiece with a 0.3 x, 0.5 x and 1 x objectives with programmable motorized zoom. Video capable for protocol development.
Cellular Imaging Core CLS 12 12145 SteREO Discovery Dissecting Microscope
Ultraview Vox Spinning Disk Confocal Perkin Elmer
Equipped with an incubator unit and automated stage, this system allows long term imaging of living samples.
Cellular Imaging Core CLS 13035 Ultraview Vox Spinning Disk Confocal
LSM 710 Multiphoton Laser Scanning Confocal Zeiss
This instrument has a MaiTai tuneable IR laser and 2 channel GaAsP non-descanned detector for multiphoton imaging. Laser lines include 453, 488, 514, 561, 594 and 633.
Cellular Imaging Core CLS 13 13035 LSM 710 Multiphoton Laser Scanning Confocal
LSM 700 Laser Scanning Confocal Zeiss
This upright confocal is particularly suitable for combinations of GFP/YFP with the new generation of fluorescent proteins (e.g. mOrange/mCherry), and is fitted with a manual stage, facilitating easy location of samples/regions of interest.
Cellular Imaging Core CLS 13 13035

LSM 700 Laser Scanning Confocal

3-Laser MACSVYB (Analyzer) Miltenyi MACSVYB
The MACSQuant VYB is a versatile 3 laser, 10-parameter flow cytometer that has enhanced capabilities for detection of fluorescent protein reporters. It is a fully operated instrument capable of running multi-tube, 96 and 384 plates. 
Flow Cytometry Karp RM 8018 3-Laser MACSVYB
ADVIA 2120 Hematology Analyzer Sysmex Advia 2120
The ADVIA is a hematology analyzer for measure blood count. It provides the flexibility of measuring multiple diff species and complete blood count with differential and reticulocyte count. 
Flow Cytometry Karp RM 8018
ADVIA 2120 Hematology Analyzer
6-Laser FACSAria (Sorter) BD FACSARIA II
This instrument is capable of measuring 15 parameters, providing higher flexibility in experiment design for sorting. It is equipped with a 561 and 449 Laser line permitting detecting of Mcherry and cyan protein. This instrument provide all the great features we come to expect with the FACSAria sorters such as customized speed, multi population, variety of collection vessels and index sorting.
Molecular Genetics Core Karp 8 RM 8113 6-Laser FACSAria
MACS Quant VYB MACS QUANT ANALYZER
MACSVYB is an air-cooled three-laser benchtop flow Cytometer analyzer. It provides our users with the ability to measure up to 8 colors + 2 Scatters It is equipped with high throughput sampler that provides rapid, fully automated sample acquisition. They are compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates
Flow Cytometry Karp RM 08016

MACS Quant VYB

4 Laser ARIA FUSION BD FACS FUSION
This Aria is located in CLS building room 15037. It is inside a self-contain hood allowing for sorting of Primary Human in a complete sterile environment. The CLS-Aria is a bench-top high-speed sorter with fixed- alignment cuvette flow cell for high fluorescence sensitivity.
Flow Cytometry Karp RM 6113 4 Laser ARIA FUSION
4 Laser CLS-ARIA BD FACS ARIA II
This Aria is located in CLS building room 15037. It is inside a self-contain hood allowing for sorting of Primary Human in a complete sterile environment. The CLS-Aria is a bench-top high-speed sorter with fixed- alignment cuvette flow cell for high fluorescence sensitivity.
Flow Cytometry CLS 15 15037 4 Laser CLS-ARIA
6 Laser Self-Aria BD FACS ARIA II
This Aria can be operated by our staff or users who have been trained and certified by our facility. It is bench-top high-speed sorter with fixed- alignment cuvette flow cell for high fluorescence sensitivity.
Karp Rm 8113

6 Laser Self-Aria

Agilent Bioanalyzer Agilent/Bioanalyzer 2100
The 2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of biomolecules. The 2100 Bioanalyzer instrument, together with the 2100 Expert Software and Bioanalyzer assays, provide highly precise analytical evaluation of various samples types in many workflows, including next generation sequencing (NGS), gene expression, biopharmaceutical, and gene editing research. Digital data is provided in a timely manner and delivers objective assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for an accurate result, and the data may be exported in a many different formats for ease-of-use. 
Molecular Genetics Core CLS 16 16030.23 Agilent Bioanalyzer
Perkin Elmer Multilabel Counter Perkin Elmer/Victor3
Fluorescence detection for quantification using Picogreen or Ribogreen.
Molecular Genetics Core CLS 16 16030.22 Perkin Elmer Multilabel Counter
Fluidigm Biomark high-performance PCR system Fluidigm/Biomark
Microfluidics technology to process samples at nanoliter-scale volumes for gene expression, genotyping, sample identification, copy number variation analysis and digital PCR. Ideal for labs performing PCR on hundreds to thousands of samples. Provides a wide range of integrated fluidic circuit (IFC) formats available, scaling from mid- to high throughput on a single system is easily achievable. Available controller are HX, MX, and RX.
Molecular Genetics Core CLS 16 16030.21 Fluidigm Biomark high-performance PCR system